CD50 Antibody | Bu65

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CD50 Antibody | Bu65 gallery image 1

Staining of human peripheral blood monocytes with Mouse anti Human CD50 (MCA2658)

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CD50 Antibody | Bu65 gallery image 2

Staining of human peripheral blood monocytes with Mouse anti Human CD50:FITC (MCA2658F)

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  • CD50 Antibody | Bu65 thumbnail image 1
  • CD50 Antibody | Bu65 thumbnail image 2
  • Mouse anti Human CD50
  • Mouse anti Human CD50:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    Bu65
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2658FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2658F
    MCA2658F, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA2658
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD50 antibody, clone Bu65 recognizes human CD50, also known as Inter-cellular adhesion molecule-3 (ICAM-3). CD50 is an 110kD type I transmembrane glycoprotein which is expressed mainly on resting leukocytes. It interacts with lymphocyte function-associated antigen-1 (LFA-1) and DC-SIGN, and is involved in the intercellular adhesion of leukocytes and augments CD3 signalling in T-lymphocyte activation.

      CD50 expression induces cancer cell proliferation and over-expression may contribute to cancer progression. It has also been implicated in limiting HIV-1 replication.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      integrin binding
      cell-cell adhesion
      integral to plasma membrane
      regulation of immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/200
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD50 Antibody Formats

    Formats Clone Applications Sizes available
    CD50 Antibody : FITC Bu65 F 0.1 mg
    CD50 Antibody : Purified Bu65 F, IP 0.2 mg
    • Copyright © 2016 Bio-Rad

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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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