CD49d Antibody

//www.bio-rad-antibodies.com/human-cd49d-antibody-ahp1225.html
CD49d Antibody gallery image 1

Staining of formalin fixed, paraffin embedded human thymic medulla with Rabbit anti Human CD49d (AHP1225)

Enlarge
CD49d Antibody gallery image 2

Published customer image:
Rabbit anti Human CD49d antibody used for immunohistochemistry on paraffin embedded sections
Image caption:
Human BM infiltration and treatment-free survival analysis of CLL patients with discordant VLA-4 and CD38 risk. (A) Human bone marrow biopsy histology was used to determine the BM lymphoid infiltrate rate of the individual VLA-4/CD38 discordant patients (n = 15). “High”: lymphoid infiltration of human BM greater than 50%, “Low”: = 50%. (B) Kaplan-Meier analysis of treatment-free survival of CLL patients (n = 144) separated by their VLA-4 and CD38 risk (VLA-4-/CD38-, orange line, n = 83; VLA-4+/CD38+, green line, n = 30; VLA-4-/CD38+, blue line, n = 13; VLA-4+/CD38-, red line, n = 18; Logrank test for trend compared to VLA-4-CD38- control-group, p = .0004). When comparing the individual survival curves of all patient groups, all three groups with individual or combined high-risk parameters show significantly shorter treatment free-survival than patient group with VLA-4-/CD38- (VLA-4+/CD38+, p = .0042; VLA-4-/CD38+, p<.0001, VLA-4+/CD38-, p = .0026; Logrank (Mantel-Cox) test, adjusted significance level for multiple comparisions of survival curves by Bonferroni method = 0.0083). There is no significant difference in treatment-free survival between the three high-risk groups (VLA-4+/CD38+ vs. VLA-4-/CD38+, p = .1861; VLA-4+/CD38+ vs. VLA-4+/CD38-, p = .9346; VLA-4-/CD38+ vs. VLA-4+/CD38-, p = .2350; Logrank (Mantel-Cox) test, adjusted significance level for multiple comparisions of survival curves by Bonferroni method = 0.0083).

From: Brachtl G, Sahakyan K, Denk U, Girbl T, Alinger B, et al. (2011) Differential Bone Marrow Homing Capacity of VLA-4 and CD38 High Expressing Chronic Lymphocytic Leukemia Cells. PLoS ONE 6(8): e23758.

Enlarge
  • CD49d Antibody thumbnail image 1
  • CD49d Antibody thumbnail image 2
  • Rabbit anti Human CD49d (C-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1225P *datasheet pdfdatasheet pdf50 µg
    AHP1225
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human CD49d polyclonal antibody detects an epitope within the C-terminal region of human CD49d, also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer’s patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997).

      VLA-4 is expressed on lymphocytes, monocytes, mast cells, macrophages, basophils and eosinophils, and has key roles in inflammation, cellular adhesion and cellular activation. LPAM-1 is expressed mainly on lymphocytes, and plays a key role in lymphocyte recruitment to sites of inflammation.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human CD49d were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.1%Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide: C-terminal domain of human CD49d, conjugated to KLH.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      receptor activity
      B cell differentiation
      identical protein binding
      leukocyte cell-cell adhesion
      leukocyte migration
      integrin-mediated signaling pathway
      regulation of immune response
      integrin complex
      blood coagulation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)1525ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Thymus, Medulla.
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD49d Antibody Formats

    Formats Applications Sizes available
    CD49d Antibody : Purified P * 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Histar Detection SystemSTAR3000C500 TestsC, P
          STAR3000C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Thymus, Medulla.

              References

              Further Reading

              Write your review

              You may also be interested in...

              You May Also Be Interested In...