CD45RO Antibody | UCHL1

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CD45RO Antibody | UCHL1 gallery image 1

Figure A. FITC conjugated mouse anti human CD45RA (MCA88F) and RPE-Alexa647® conjugated Mouse IgG2a isotype control (MCA929A647). Figure B. FITC conjugated mouse anti human CD45RA (MCA88F) and RPE-Cy5 conjugated mouse anti human CD45RO (MCA461C). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A)

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  • CD45RO Antibody | UCHL1 thumbnail image 1
  • Mouse anti Human CD45RO
  • Mouse anti Human CD45RO
  • Mouse anti Human CD45RO:RPE-Cy5
  • Mouse anti Human CD45RO:FITC
  • Mouse anti Human CD45RO
  • Mouse anti Human CD45RO:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    UCHL1
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA461GAC, F, IP, Pdatasheet pdfdatasheet pdf0.1 mg
    MCA461GA
    MCA461FFdatasheet pdfdatasheet pdf0.1 mg
    MCA461F
    MCA461GC, F, IP, Pdatasheet pdfdatasheet pdf0.2 mg
    MCA461G
    MCA461CFdatasheet pdfdatasheet pdf100 Tests/0.5ml
    MCA461C
    MCA461FTFdatasheet pdfdatasheet pdf25 µg
    MCA461FT
    MCA461GTC, F, IP, Pdatasheet pdfdatasheet pdf25 µg
    MCA461GT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti-human CD45RO monoclonal antibody, clone UCHL1 recognizes the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype.

      In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Chimpanzeeyes
      Marmosetyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to RPE-Cy5 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Human IL-2 dependent T cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      6 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      focal adhesion
      membrane raft
      release of sequestered calcium ion into cytosol
      integral to plasma membrane
      negative regulation of T cell mediated cytotoxicity
      immunoglobulin biosynthetic process
      B cell receptor signaling pathway
      transmembrane receptor protein tyrosine phosphatase activity
      positive regulation of B cell proliferation
      B cell proliferation
      protein kinase binding
      positive regulation of antigen receptor-mediated signaling pathway
      defense response to virus
      protein dephosphorylation
      regulation of S phase
      negative regulation of cytokine-mediated signaling pathway
      negative regulation of protein kinase activity
      axon guidance
      T cell receptor signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Cy® and CyDye® are registered trademarks of GE Healthcare
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/50
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/50
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/50
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Immunohistology
    • Immunohistology
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Immunohistology
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD45RO Antibody Formats

    Formats Clone Applications Sizes available
    CD45RO Antibody : Purified UCHL1 C, F, IP, P 0.1 mg | 0.2 mg | 25 µg
    CD45RO Antibody : FITC UCHL1 F 0.1 mg | 25 µg
    CD45RO Antibody : RPE-Cy5 UCHL1 F 100 Tests/0.5ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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              Tonsil
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              • Application NameReference Images
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              References

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