Mouse anti Human CD45RA:FITC/CD8:RPE has been developed to allow the simulatneous evaluation of CD45RA and CD8 expressing cells by flow cytometry.
CloneF8-11-13 recognises CD45RA, a ~220 kDa glycoprotein expressed by B cells, monocytes and a subset of T-cells.Clone LT8 recognises CD8, a 32kD glycoprotein expressed by the suppressor/cytotoxic subset of T cells, and weakly by NK cells.
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.