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CD45RA/CD62L/CD3/CD8 | F8-11-13 / FMC46 / UCHT1 / LT8

CD45RA/CD62L/CD3/CD8 | F8-11-13 / FMC46 / UCHT1 / LT8 gallery image 1

Human peripheral blood lymphocytes stained with Multi-Colour Human CD45RA/CD62L/CD3/CD8 flow kit

  • CD45RA/CD62L/CD3/CD8 | F8-11-13 / FMC46 / UCHT1 / LT8 thumbnail image 1
  • Four-Color Human CD45RA/CD62L/CD3/CD8 Flow Kit
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  • Product Type
    Four Color Reagent
  • Clone
    F8-11-13 / FMC46 / UCHT1 / LT8
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4C001Fdatasheet pdfdatasheet pdf50 Tests/0.5ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Four-Color Human CD45RA/CD62L/CD3/CD8 Flow Kit can be used for single-step identification of suppressor/cytotoxic naïve T-cells (CD3+CD8+CD45RA+CD62L+) and suppressor/cytotoxic memory T-cell subsets (CD3+CD8+CD45RA+CD62L-, CD3+CD8+CD45RA-CD62L- or CD3 +CD8+CD45RA-CD62L+).

      CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ), consisting of two heterodimers (εγ, εδ) and one homodimer (ζζ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the 20kDa CD3ε chain.

      CD8 is a cell surface glycoprotein which acts as a co-receptor for MHC Class I, in conjunction with the T-cell receptor (TCR). CD8 exists as a dimer, composed of two α chains or more commonly as an αβ heterodimer. The CD8 antigen is expressed on the human suppressor/cytotoxic T-cell subset (CD3+CD8+) and on a subset of NK cells. Binding of CD8 to MHC class I, acts to enhance resting T-cell activation. Clone LT8 is specific for the CD8α chain.

      CD45RA is an isoform of CD45, a member of the protein tyrosine phosphatase family, otherwise known as leucocyte common antigen (LCA), which acts as a positive regulator of T-cell co-activation, and is essential for the activation of T-cells through the antigen receptor. CD45RA is expressed by naïve T-cells and is down-regulated during activation.

      CD62L is a cell surface adhesion protein, and member of the selectin/LECAM family, otherwise known as leukocyte-endothelial cell adhesion molecule 1 (LECAM1), which acts as a mediator of lymphocyte homing to high endothelial venules (HEVs), within peripheral lymphoid tissues. CD62L is constitutively expressed by all leucocytes including naïve T-cells, but only by certain subpopulations of memory T-cells. Levels of serum CD62L can be used as an indicator of inflammation.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD45RA/CD62L/CD3/CD8 Formats

    Formats Clone Applications Sizes available
    CD45RA/CD62L/CD3/CD8 : 4 Color F8-11-13 / FMC46 / UCHT1 / LT8 F 50 Tests/0.5ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Flow Cytometry Absolute Count Standard™FCSC58020 TestsF
          Erythrolyse Red Blood Cell Lysing Buffer (10x)BUF04B100 Test/20mlF
          Human SeroblockBUF070B200 TestF
          Human SeroblockBUF070A50 TestF
          Erythrolyse Red Blood Cell Lysing Buffer (10x)BUF04C500 Tests/100mlF

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • CD45RA/CD62L/CD3/CD8 | F8-11-13 / FMC46 / UCHT1 / LT8 thumbnail image 1

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