CD45RA/CD45RO/CD8/CD3 | F8-11-13 / UCHL1 / LT8 / UCHT1

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CD45RA/CD45RO/CD8/CD3 | F8-11-13 / UCHL1 / LT8 / UCHT1 gallery image 1

Human peripheral blood lymphocytes stained with Multi-Colour Human CD45RA/CD45RO/CD8/CD3 flow kit (4C005)

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  • CD45RA/CD45RO/CD8/CD3 | F8-11-13 / UCHL1 / LT8 / UCHT1 thumbnail image 1
  • Four-Color Human CD45RA/CD45RO/CD8/CD3 Flow Kit
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  • Product Type
    Four Color Reagent
  • Clone
    F8-11-13 / UCHL1 / LT8 / UCHT1
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4C005Fdatasheet pdfdatasheet pdf50 Tests/0.5ml
    4C005
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Four-Color Human CD45RA/CD45RO/CD8/CD3 Flow Kit can be used for single-step identification of human suppressor/cytotoxic naïve (CD3+CD8+CD45RA+) and suppressor/cytotoxic memory (CD3+CD8+CD45RO+) T-cell subsets.

      CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ), consisting of two heterodimers (εγ, εδ) and one homodimer (ζζ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the ~20 kDa CD3ε chain.

      CD8 is a cell surface glycoprotein which acts as a co-receptor for MHC Class I, in conjunction with the T-cell receptor (TCR). CD8 exists as a dimer, composed of two α chains or more commonly as an αβ heterodimer. The CD8 antigen is expressed on the human suppressor/cytotoxic T-cell subset (CD3+CD8+) and on a subset of NK cells. Binding of CD8 to MHC class I, acts to enhance resting T-cell activation. Clone LT8 is specific for the CD8α chain.

      CD45RA is an isoform of CD45, a member of the protein tyrosine phosphatase family, otherwise known as leucocyte common antigen (LCA), which acts as a positive regulator of T-cell co-activation, and is essential for the activation of T-cells through the antigen receptor. CD45RA is expressed by naïve T-cells, but not by activated and memory T-cells. Consequently, clone F8-11-13 can be used to distinguish between these cell populations.

      CD45RO is the low molecular weight isoform (~180 kDa) of CD45, the leucocyte common antigen (LCA). CD45RO is expressed by approximately 40% of resting peripheral blood T-cells, the majority of activated and memory T-cells and most thymocytes. It is also expressed on macrophages, monocytes and granulocytes. In combination with clone F8-11-13, clone UCHL1 can be used to discern naïve and memory T-cells.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD45RA/CD45RO/CD8/CD3 Formats

    Formats Clone Applications Sizes available
    CD45RA/CD45RO/CD8/CD3 : 4 Color F8-11-13 / UCHL1 / LT8 / UCHT1 F 50 Tests/0.5ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Flow Cytometry Absolute Count Standard™FCSC58020 TestsF
          FCSC580
          Erythrolyse Red Blood Cell Lysing Buffer (10x)BUF04B100 Test/20mlF
          BUF04B
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          Erythrolyse Red Blood Cell Lysing Buffer (10x)BUF04C500 Tests/100mlF
          BUF04C

          Recommended Positive Controls

            Histology Controls

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