CD45RA Antibody | HI100

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  • Mouse anti Human CD45RA
  • Mouse anti Human CD45RA:RPE
  • Mouse anti Human CD45RA:RPE-Cy5
  • Mouse anti Human CD45RA:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HI100
  • Isotype
    IgG2b
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2811GAFdatasheet pdfdatasheet pdf0.1 mg
    MCA2811GA
    MCA2811FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2811F
    MCA2811PEFdatasheet pdfdatasheet pdf100 Tests/0.5ml
    MCA2811PE
    MCA2811CFdatasheet pdfdatasheet pdf100 Tests/0.5ml
    MCA2811C
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD45RA antibody, clone HI100 recognizes the human CD45RA cell surface antigen, the 220 kDa exon 4 splice variant of the leukocyte common antigen, also known as CD45, LCA or receptor-type tyrosine-protein phosphatase C. CD45 is a single pass type I transmembrane glycoprotein with two fibronectin type III domains and two tyrosine-protein phosphatase domains (UniProt: P08575). CD45RA is expressed by a subset of T lymphocytes, B lymphocytes and most monocytes.

      Mutations in the CD45 gene can lead to severe combined immunodeficiency disease characterised by a complete lack of the T cell mediated immune response (Tchilian et al. 2001) and a susceptibility for the development of multiple sclerosos (Jacobsen et al. 2000).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Chimpanzeeyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to RPE - Cy5 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 0.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline.
      Phosphate buffered saline.
      Phosphate buffered saline.
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch
      6 months from date of despatch.
      12 months from date of despatch
    • GO Terms
      positive regulation of antigen receptor-mediated signaling pathway
      protein kinase binding
      integral to plasma membrane
      positive regulation of B cell proliferation
      B cell proliferation
      release of sequestered calcium ion into cytosol
      membrane raft
      regulation of S phase
      focal adhesion
      negative regulation of cytokine-mediated signaling pathway
      defense response to virus
      axon guidance
      T cell receptor signaling pathway
      transmembrane receptor protein tyrosine phosphatase activity
      protein dephosphorylation
      negative regulation of T cell mediated cytotoxicity
      B cell receptor signaling pathway
      negative regulation of protein kinase activity
      immunoglobulin biosynthetic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Cy® and CyDye® are registered trademarks of GE Healthcare
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD45RA Antibody Formats

    Formats Clone Applications Sizes available
    CD45RA Antibody : Purified HI100 F 0.1 mg
    CD45RA Antibody : RPE-Cy5 HI100 F 100 Tests/0.5ml
    CD45RA Antibody : FITC HI100 F 0.1 mg
    CD45RA Antibody : RPE HI100 F 100 Tests/0.5ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
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      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2b:FITCHCA038F0.1 mgF
      HCA038F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
      HCA038P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:RPEMCA691PE100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        MCA691C
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:FITCMCA691F100 TestsF
        MCA691F

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

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