CD45RA Antibody | HI100

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  • Mouse anti Human CD45RA:RPE-Cy5
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  • Product Type
    Monoclonal Antibody
  • Clone
    HI100
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2811CFdatasheet pdfdatasheet pdf100 Tests/0.5ml
    MCA2811C
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CD45RA antibody, clone HI100 recognises the human CD45RA cell surface antigen, the 220 kDa exon 4 splice variant of the leucocyte common antigen, also known as CD45, LCA or Receptor-type tyrosine-protein phosphatase C. CD45 is a single pass type I transmembrane glycoprotein with two fibronectin type III domains and two tyrosine-protein phosphatase domains (UniProt: P08575). CD45RA is expressed by a subset of T lymphocytes, B lymphocytes and most monocytes.

      Mutations in the CD45 gene can lead to sever combined immunodeficiency disease characterised by a complete lack of the T cell mediated immune response (Tchilian et al. 2001) and a susceptibility for the development of multiple sclerosos (Jacobsen et al. 2000).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 5ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD45RA Antibody Formats

    Formats Clone Applications Sizes available
    CD45RA Antibody : RPE-Cy5 HI100 F 100 Tests/0.5ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:RPE-Cy5MCA691C100 TestsF
        MCA691C

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              References

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