Mouse anti Human CD45RA antibody, clone Bu78 recognizes human CD45RA, a 220kD isoform of the leucocyte common antigen CD45, which is a member of the protein tyrosine phosphatase family. CD45RA is expressed by a subset of T lymphocytes, B lymphocytes and most monocytes. CD45RA plays an important role in the regulation of T- and B-cell antigen receptor signalling.
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Sodium Azide (NaN3)
Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.