CD45RA Antibody | Bu78

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  • Mouse anti Human CD45RA:FITC
  • Mouse anti Human CD45RA:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    Bu78
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2508FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2508F
    MCA2508FTFdatasheet pdfdatasheet pdf25 µg
    MCA2508FT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD45RA antibody, clone Bu78 recognizes human CD45RA, a 220kD isoform of the leucocyte common antigen CD45, which is a member of the protein tyrosine phosphatase family. CD45RA is expressed by a subset of T lymphocytes, B lymphocytes and most monocytes. CD45RA plays an important role in the regulation of T- and B-cell antigen receptor signalling.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      positive regulation of antigen receptor-mediated signaling pathway
      immunoglobulin biosynthetic process
      protein kinase binding
      protein dephosphorylation
      positive regulation of B cell proliferation
      focal adhesion
      release of sequestered calcium ion into cytosol
      B cell proliferation
      membrane raft
      T cell receptor signaling pathway
      negative regulation of protein kinase activity
      B cell receptor signaling pathway
      integral to plasma membrane
      negative regulation of cytokine-mediated signaling pathway
      transmembrane receptor protein tyrosine phosphatase activity
      defense response to virus
      negative regulation of T cell mediated cytotoxicity
      regulation of S phase
      axon guidance
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD45RA Antibody Formats

    Formats Clone Applications Sizes available
    CD45RA Antibody : FITC Bu78 F 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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