CD45 Antibody | 135-4C5
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Enlarge- Mouse anti Human CD45
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Product TypeMonoclonal Antibody
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Clone135-4C5
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IsotypeIgG2b
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti Human CD45 antibody, clone 135-4C5 recognises the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Mouse anti Human CD45 antibody, clone 135-4C5 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.
This product is routinely tested in flow cytometry on human peripheral blood leucocytes.
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Intended Use
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Target SpeciesHuman
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Product FormPurified IgG - liquid
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Reconstitution
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PreparationPurified IgG prepared by affinity chromatography on Protein G
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Preservative Stabilisers0.09% Sodium Azide (NaN3)
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Purity
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Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered saline
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StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.
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GO Termspositive regulation of antigen receptor-mediated signaling pathwayprotein kinase bindingmembrane raftrelease of sequestered calcium ion into cytosolB cell proliferationnegative regulation of T cell mediated cytotoxicityintegral to plasma membranenegative regulation of protein kinase activitynegative regulation of cytokine-mediated signaling pathwayB cell receptor signaling pathwaydefense response to virusfocal adhesionpositive regulation of B cell proliferationprotein dephosphorylationtransmembrane receptor protein tyrosine phosphatase activityimmunoglobulin biosynthetic processT cell receptor signaling pathwayregulation of S phaseaxon guidance
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Flow Cytometry 1/10 1/100 Immunofluorescence Immunohistology - Frozen 1/200 Western Blotting Immunohistology - Paraffin(1) 1/200 (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.- Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- Flow CytometryUse 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- ELISA
- Immunohistology
- Histology Positive Control TissueHuman tonsil
- Immunofluorescence
- Western BlottingMCA2727 detects a band/s of approximately 180kDa, 200kDa, 210kDa and 220kDa in B-cell lysates.
- Instructions For Use
Additional CD45 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| CD45 Antibody : Purified | 135-4C5 | C, F, IF, P*, WB | 0.2 mg |
- Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
Recommended Negative Isotype Control
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | 100 Tests | F | MCA691 |
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Histar Detection System | STAR3000B | 150 Tests | C, P | STAR3000B | |
| Histar Detection System | STAR3000A | 50 Tests | C, P | STAR3000A | |
| Histar Detection System | STAR3000C | 500 Tests | C, P | STAR3000C |
Recommended Positive Controls
Histology Controls
Human tonsil
Application Name Reference Images Immunofluorescence
References
1. Alsinet, E. et al. (1990) Differential effects of anti-CD45 monoclonal antibody on human B cell proliferation: a monoclonal antibody recognizing a neuraminidase-sensitive epitope of the T200 molecule enhances anti-immunoglobulin-induced proliferation.Eur J Immunol. 20 (12): 2801-4.
2. Thiele, G.M. et al. (2015) Malondialdehyde-acetaldehyde adducts and anti-malondialdehyde-acetaldehyde antibodies in rheumatoid arthritis.Arthritis Rheumatol. 67 (3): 645-55.
3. Gómez, M.C. et al. (2015) Characterization and Multilineage Differentiation of Domestic and Black-Footed Cat Mesenchymal Stromal/Stem Cells from Abdominal and Subcutaneous Adipose Tissue.Cell Reprogram. 17 (5): 376-92.
