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CD44v7 antibody | VFF-9

Product Code Applications Pack Size List Price Your Price Qty
MCA1731
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * WB 0.1 mg loader
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loader

Mouse anti Human CD44v7 antibody, clone VFF-9 specifically recognizes an epitope encoded by exon v7 on the variant portion of human CD44.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3X63Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50
Immunohistology - Frozen 1/50
Immunohistology - Paraffin 1 1/50
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl
Histology Positive Control Tissue
Human skin

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Source Reference

  1. Koopman, G. et al. (1993) Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.
    J Exp Med. 177 (4): 897-904.

References for CD44v7 antibody

  1. Bànkfalvi, A. et al. (1998) Gains and losses of CD44 expression during breast carcinogenesis and tumour progression.
    Histopathology. 33 (2): 107-16.
  2. Hanley, W.D. et al. (2005) Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cells.
    FASEB J. 20: 337-9.
  3. Kim, J.S. and Werth, V.P. (2011) Identification of specific chondroitin sulfate species in cutaneous autoimmune disease.
    J Histochem Cytochem. 59: 780-90.
  4. Rajarajan, A. et al. (2012) CD44 expression in oro-pharyngeal carcinoma tissues and cell lines.
    PLoS One. 7: e28776.
  5. Kim, J.S. et al. (2012) Gottron's papules exhibit dermal accumulation of CD44 variant 7 (CD44v7) and its binding partner osteopontin: a unique molecular signature.
    J Invest Dermatol. 132 (7): 1825-32.
  6. Shirure, V.S. et al. (2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.
    Am J Physiol Cell Physiol. 308 (1): C68-78.
  7. Noori, M.S. et al. (2018) An adhesion based approach for the detection of esophageal cancer.
    Integr Biol (Camb). 10 (12): 747-57.

Flow Cytometry

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

RRID
AB_322695
UniProt
P16070
Entrez Gene
CD44

MCA1731

153636 158584 167361

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