CD44 Antibody | F10-44-2

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CD44 Antibody | F10-44-2 gallery image 1

Staining of human peripheral blood granulocytes with Mouse anti Human CD44:Alexa Fluor® 647 (MCA89A647)

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CD44 Antibody | F10-44-2 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD44:Biotin (MCA89B)

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CD44 Antibody | F10-44-2 gallery image 3

Staining of human peripheral blood lymphocytes with Mouse anti Human CD44:FITC (MCA89F)

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CD44 Antibody | F10-44-2 gallery image 4

Staining of human peripheral blood lymphocytes with Mouse anti Human CD44:RPE (MCA89PE)

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CD44 Antibody | F10-44-2 gallery image 5

Staining of human peripheral blood lymphocytes with Mouse anti Human CD44:Azide free (MCA89XZ)

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CD44 Antibody | F10-44-2 gallery image 6

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human CD44 antibody (MCA89) followed by HRP-polymer detection and DAB substrate development (low power) following antigen retrieval using citrate buffer at pH6.2

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CD44 Antibody | F10-44-2 gallery image 7

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human CD44 antibody (MCA89) followed by HRP-polymer detection and DAB substrate development (high power) following antigen retrieval using citrate buffer at pH6.2

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  • CD44 Antibody | F10-44-2 thumbnail image 1
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  • CD44 Antibody | F10-44-2 thumbnail image 7
  • Mouse anti Human CD44:RPE
  • Mouse anti Human CD44
  • Mouse anti Human CD44:RPE
  • Mouse anti Human CD44:Alexa Fluor® 647
  • Mouse anti Human CD44:Alexa Fluor® 647
  • Mouse anti Human CD44:FITC
  • Mouse anti Human CD44:FITC
  • Mouse anti Human CD44
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    F10-44-2
  • Isotype
    IgG2a
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA89FFdatasheet pdfdatasheet pdf0.1 mg
    MCA89F
    MCA89C, F, IF, Pdatasheet pdfdatasheet pdf0.2 mg
    MCA89
    MCA89PEFdatasheet pdfdatasheet pdf100 Tests
    MCA89PE
    MCA89A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA89A647
    MCA89TC, F, IF, Pdatasheet pdfdatasheet pdf20 µg
    MCA89T
    MCA89FTFdatasheet pdfdatasheet pdf25 µg
    MCA89FT
    MCA89PETFdatasheet pdfdatasheet pdf25 Tests
    MCA89PET
    MCA89A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA89A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD44 antibody, clone F10-44-2 recognizes human CD44, also known as Epican, HCAM, Phagocytic Glycoprotein 1 (PGP-1) or Hyaluronate receptor . Human CD44 is a single pass, type I transmembrane glycoprotein of variable molecular weight ranging from ~90kDa to ~220kDa depending on alternate splicing of the variable region exons and on the degree of glycosylation. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor (Laurent and Fraser 1992).

      CD44 isoforms containing one or more sequences encoded by the variant region exons have a much more restricted expression pattern both in terms of organ specificity and immune activation (Mackay et al. 1994). .

      Mouse anti Human CD44 antibody, clone F10-44-2 recognizes an epitope on human CD44 outside the regions coded for by the variable region exons and is thus expected to recognize all isoforms of human CD44 (Mackay et al. 1994).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Cynomolgus monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Human T lymphocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells of immunised BALB/c mice were fused with cells from the mouse NS1 myeloma line.
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      This product should be stored undiluted.
      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      This product should be stored undiluted.
      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/20
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin1/501/100
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/20
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin1/501/100

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human Tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human Tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD44 Antibody Formats

    Formats Clone Applications Sizes available
    CD44 Antibody : FITC F10-44-2 F 0.1 mg | 25 µg
    CD44 Antibody : Purified F10-44-2 C, F, IF, P 0.2 mg | 20 µg
    CD44 Antibody : RPE F10-44-2 F 100 Tests | 25 Tests
    CD44 Antibody : Alexa Fluor® 647 F10-44-2 F 25 Tests/0.25ml | 100 Tests/1ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              Human Tonsil
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              References

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