CD42a Antibody | FMC-25

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CD42a Antibody | FMC-25 gallery image 1

Human peripheral blood platelets stained with Mouse anti Human CD42a:FITC (MCA594F)

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  • CD42a Antibody | FMC-25 thumbnail image 1
  • Mouse anti Human CD42a:FITC
  • Mouse anti Human CD42a:RPE
  • Mouse anti Human CD42a
  • Mouse anti Human CD42a:FITC
  • Mouse anti Human CD42a
  • Mouse anti Human CD42a:RPE
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  • Product Type
    Monoclonal Antibody
  • Clone
    FMC-25
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA594GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA594GA
    MCA594FFdatasheet pdfdatasheet pdf0.1 mg
    MCA594F
    MCA594C, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA594
    MCA594PEFdatasheet pdfdatasheet pdf100 Tests
    MCA594PE
    MCA594FTFdatasheet pdfdatasheet pdf25 µg
    MCA594FT
    MCA594PETFdatasheet pdfdatasheet pdf25 Tests
    MCA594PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD42a antibody, clone FMC-25 recognizes human CD42a, also known as Platelet glycoprotein IX, Glycoprotein 9 or GP-IX. CD42a is a 177 amino acid, ~20kDa type I single pass transmembrane glycoprotein containing a single leucine-rich repeat containing N-terminal domain and a single leucine-rich repeat containing C-terminal domain.

      CD42a is expressed by platelets and megakaryocytes and forms a covalent complex with CD42c (GP-1b-beta), CD42b (GP-1b-alpha) and CD42d (platelet glycoprotein V) to create the platelet surface receptor for von Willebrand factor. Incubation of the intact von Willebrand receptor complex with clone FMC-25 does not appear to inhibit binding of von Willebrand factor to the receptor (Yan et al. 2011). Defects in the GP1BB gene encoding human CD42a can lead to the inherited bleeding disorder Bernard-Soulier syndrome (Diz-Küçükkaya 2013), characterized by prolonged bleeding times, thrombocytopenia and the appearence of giant platelets in the circulation (Johns et al. 2014).

      Mouse anti human CD42a antibody, clone FMC-25 has been successfully used as a capture reagent for platelet-autoantibody complexes in the sera of patients presenting thrombocytopenia associated with anti-phospholipid syndrome (Godeau et al. 1997).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
      Reconstitute with 1.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Peripheral blood mononuclear cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
      Pack Size: 0.1 mgIgG concentration 1.0mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
      IgG concentration 0.1mg/ml
      Pack Size: 0.1 mgIgG concentration 1.0mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline.
      Phosphate buffered saline.
      Phosphate buffered saline
      Phosphate buffered saline.
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      integral to plasma membrane
      protein binding
      platelet activation
      blood coagulation, intrinsic pathway
      cell adhesion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeatNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeatNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Bone marrow
    • Histology Positive Control Tissue
      Bone marrow
    • Histology Positive Control Tissue
      Bone marrow
    • Histology Positive Control Tissue
      Bone marrow
    • Histology Positive Control Tissue
      Bone marrow
    • Histology Positive Control Tissue
      Bone marrow
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD42a Antibody Formats

    Formats Clone Applications Sizes available
    CD42a Antibody : RPE FMC-25 F 100 Tests | 25 Tests
    CD42a Antibody : FITC FMC-25 F 25 µg | 0.1 mg
    CD42a Antibody : Purified FMC-25 C, F 0.25 mg | 0.1 mg
    • Copyright © 2016 Bio-Rad

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          Recommended Positive Controls

            Histology Controls

              Bone marrow
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              • Application NameReference Images
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              References

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