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CD40 Antibody | 3B2

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Sandwich ELISA analysis of Human CD40 binding using Mouse anti Human CD40 antibody, clone 3B2 (MCA2788GA) as a capture reagent and biotinylated Mouse anti Human CD40 antibody, clone LOB7/6 (MCA1590) as a detection reagent with purified Human CD40 as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Undiluted serum and plasma samples are shown green and orange respectively.

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  • Mouse anti Human CD40

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  • Product Type
    Monoclonal Antibody
  • Clone
    3B2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2788GAC, E, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2788GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD40 antibody, clone 3B2 recognizes human CD40, a type I transmembrane glycoprotein receptor and member of the TNF-receptor superfamily, primarily expressed by B cells and professional antigen-presenting cells (APCs), which plays a vital role in T cell-B cell interactions and in germinal centre B cell selection.

      CD40 is a co-stimulatory molecule which mediates its immunoregulatory effects through interaction with its ligand CD154 (CD40L, gp39), playing a role in B cell proliferation and memory development, Ig class switching, activation of APCs and subsequent cytokine production, and the expansion of antigen-specific CD4+ T cells.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1.0ug/ml
      Immunohistology - Frozen10.0ug/ml
      Western Blotting10.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD40 Antibody Formats

    Formats Clone Applications Sizes available
    CD40 Antibody : Purified 3B2 C, E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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          Recommended Positive Controls

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              References

              1. Contin, C. et al. (2003) Membrane-anchored CD40 is processed by the tumor necrosis factor-alpha-converting enzyme. Implications for CD40 signaling.
              J. Biol. Chem. 278: 32801-32809.

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