CD40 Antibody | 3B2

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Sandwich ELISA analysis of Human CD40 binding using Mouse anti Human CD40 antibody, clone 3B2 (MCA2788GA) as a capture reagent and biotinylated Mouse anti Human CD40 antibody, clone LOB7/6 (MCA1590) as a detection reagent with purified Human CD40 as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Undiluted serum and plasma samples are shown green and orange respectively.

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  • Mouse anti Human CD40
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    • Product Type
      Monoclonal Antibody
    • Clone
      3B2
    • Isotype
      IgG1
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA2788GAC, E, WBdatasheet pdfdatasheet pdf0.1 mg
      MCA2788GA
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Mouse anti Human CD40 antibody, clone 3B2 recognizes human CD40, a type I transmembrane glycoprotein receptor and member of the TNF-receptor superfamily, primarily expressed by B cells and professional antigen-presenting cells (APCs), which plays a vital role in T cell-B cell interactions and in germinal centre B cell selection.

        CD40 is a co-stimulatory molecule which mediates its immunoregulatory effects through interaction with its ligand CD154 (CD40L, gp39), playing a role in B cell proliferation and memory development, Ig class switching, activation of APCs and subsequent cytokine production, and the expansion of antigen-specific CD4+ T cells.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA1.0ug/ml
        Immunohistology - Frozen10.0ug/ml
        Western Blotting10.0ug/ml

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional CD40 Antibody Formats

      Formats Clone Applications Sizes available
      CD40 Antibody : Purified 3B2 C, E, WB 0.1 mg

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
        STAR117A
        Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
        STAR87A
        Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
        STAR117D488GA
        Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
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        Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
        STAR117D649GA
        Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
        STAR117D680GA
        Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
        STAR117D800GA
        Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
        STAR8D800GA
        Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
        STAR117F
        Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
        STAR70
        Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
        STAR120F
        Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
        STAR9B
        Human anti Mouse IgG1:HRPHCA036P0.1 mgE
        HCA036P
        Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
        STAR117P
        Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
        STAR77
        Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
        STAR120P
        Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
        STAR13B
        Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
        STAR87P
        Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
        STAR12A
        Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
        STAR76

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Histar Detection SystemSTAR3000B150 TestsC, P
            STAR3000B

            Recommended Positive Controls

              Histology Controls

                References

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