CD40 Antibody | 3B2
Sandwich ELISA analysis of Human CD40 binding using Mouse anti Human CD40 antibody, clone 3B2 (MCA2788GA) as a capture reagent and biotinylated Mouse anti Human CD40 antibody, clone LOB7/6 (MCA1590) as a detection reagent with purified Human CD40 as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Undiluted serum and plasma samples are shown green and orange respectively.
Enlarge- Mouse anti Human CD40
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Product TypeMonoclonal Antibody
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Clone3B2
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IsotypeIgG1
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
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Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti Human CD40 antibody, clone 3B2 recognizes human CD40, a type I transmembrane glycoprotein receptor and member of the TNF-receptor superfamily, primarily expressed by B cells and professional antigen-presenting cells (APCs), which plays a vital role in T cell-B cell interactions and in germinal centre B cell selection.
CD40 is a co-stimulatory molecule which mediates its immunoregulatory effects through interaction with its ligand CD154 (CD40L, gp39), playing a role in B cell proliferation and memory development, Ig class switching, activation of APCs and subsequent cytokine production, and the expansion of antigen-specific CD4+ T cells.
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Intended Use
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Target SpeciesHuman
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Product FormPurified IgG - liquid
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Reconstitution
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PreparationPurified IgG prepared by affinity chromatography on Protein A
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Preservative Stabilisers0.09% Sodium Azide (NaN3)
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ImmunogenCD40-Fc.
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Purity
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Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered saline
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StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.
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GO TermsCD40 receptor complexB cell proliferationenzyme bindingplatelet activationextracellular regionreceptor activityinternal side of plasma membranepositive regulation of I-kappaB kinase/NF-kappaB cascadeinflammatory responseprotein complex assembly
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA 1.0ug/ml Immunohistology - Frozen 10.0ug/ml Western Blotting 10.0ug/ml - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- ELISA
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western Blotting
- Instructions For Use
Additional CD40 Antibody Formats
| Formats | Clone | Applications | Sizes available |
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| CD40 Antibody : Purified | 3B2 | C, E, WB | 0.1 mg |
Recommended Secondary Antibody
Recommended Negative Isotype Control
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Histar Detection System | STAR3000B | 150 Tests | C, P | STAR3000B |
Recommended Positive Controls
Histology Controls
References
1. Contin, C. et al. (2003) Membrane-anchored CD40 is processed by the tumor necrosis factor-alpha-converting enzyme. Implications for CD40 signaling.J Biol Chem. 278 (35): 32801-9.
