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CD4/CD8/CD3 | RPA-T4 / LT8 / S4.1

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  • anti Human CD4:FITC/CD8:RPE/CD3:RPE-Cy5
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  • Product Type
    Triple Color Reagent
  • Clone
    RPA-T4 / LT8 / S4.1
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    TC010Fdatasheet pdfdatasheet pdf50 Tests
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Anti Human CD4:FITC/CD8:RPE/CD3:RPE-CY5 may be used to simultaneously identify CD3, CD4 and CD8 positive lymphocytes.

      Clone RPA-T4 is specific for the human CD4 antigen, a 59 kDa cell surface glycoprotein expressed by the helper-inducer subset of T-cells and weakly by monocytes. LT8 recognizes the human CD8 antigen, a 32 kDa dimer expressed by a subset of T-cells with cytotoxic/suppressor activity and more weakly by NK cells. Clone S4.1 is specific for the human CD3 antigen, a pan T cell marker.
    • Intended Use
    • Target Species
    • Product Form
      Triple Color combination consisting of FITC, RPE and RPE-CY5 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
    • Reconstitution
      Reconstitute with 0.5 ml distilled water
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
    • Antibody Isotypes
      CD4 FITC reagent: Mouse IgG1
      CD8 RPE reagent: Mouse IgG1
      CD3 RPE-CY5 reagent: Mouse IgG2a
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC.


      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD4/CD8/CD3 Formats

    Formats Clone Applications Sizes available
    CD4/CD8/CD3 : 3 Color RPA-T4 / LT8 / S4.1 F 50 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          Human SeroblockBUF070A50 TestF

          Recommended Positive Controls

            Histology Controls

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