CD4/CD38/CD3/HLA DR | RPA-T4 / AT13/5 / UCHT1 / YE2/36-HLK

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CD4/CD38/CD3/HLA DR | RPA-T4 / AT13/5 / UCHT1 / YE2/36-HLK gallery image 1

Human peripheral lymphocytes stained with Multi-Colour Human CD4/CD38/CD3/HLA DR flow kit (4C006)

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  • CD4/CD38/CD3/HLA DR | RPA-T4 / AT13/5 / UCHT1 / YE2/36-HLK thumbnail image 1
  • Four-Color Human CD4/CD38/CD3/HLA DR Flow Kit
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  • Product Type
    Four Color Reagent
  • Clone
    RPA-T4 / AT13/5 / UCHT1 / YE2/36-HLK
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4C006Fdatasheet pdfdatasheet pdf50 Tests/0.5ml
    4C006
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Four-Color Human CD4/CD38/CD3/HLA DR Flow Kit can be used for single-step identification of activated helper/inducer (CD3+CD4+CD38+ and CD3+CD4+HLA DR+) T-cell subsets.

      CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ), consisting of two heterodimers (εγ, εδ) and one homodimer (ζζ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the ~20 kDa CD3ε chain.

      CD4 is a single chain transmembrane glycoprotein and member of the immunoglobulin superfamily, which interacts directly with class II molecules of the major histocompatibility complex (MHCII), and is the primary receptor for the human immunodeficiency virus (HIV). CD4 is expressed by the helper/inducer (CD3+CD4+) T-cell subset, the majority of thymocytes, and at low levels by monocytes and tissue macrophages. The CD4 lymphocyte count provides an excellent indication of immune system health. Clone RPA-T4 binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen.

      CD38, otherwise known as cyclic-ADP ribose hydrolase 1, is a type II integral transmembrane glycoprotein and member of the ADP-ribosyl cyclase family, which is widely used to study the processes of B- and T-cell differentiation and activation. Increased expression of CD38 on both CD4+ and CD8+ T-cells in HIV-infected patients is associated with disease progression.

      HLA DR is a heterodimeric cell surface glycoprotein and human class II MHC (major histocompatibility complex) cell surface receptor, consisting of a 36 kDa alpha and 27 kDa beta chain, which is essential for efficient peptide presentation to CD4+ T-cells. HLA DR is expressed primarily by antigen presenting cells and, together with CD38, is a useful marker of T-cell activation following viral infection. Clone YE2/36-HLK recognises a monomorphic determinant of human HLA DR.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD4/CD38/CD3/HLA DR Formats

    Formats Clone Applications Sizes available
    CD4/CD38/CD3/HLA DR : 4 Color RPA-T4 / AT13/5 / UCHT1 / YE2/36-HLK F 50 Tests/0.5ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Flow Cytometry Absolute Count Standard™FCSC58020 TestsF
          FCSC580
          Erythrolyse Red Blood Cell Lysing Buffer (10x)BUF04B100 Test/20mlF
          BUF04B
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          Erythrolyse Red Blood Cell Lysing Buffer (10x)BUF04C500 Tests/100mlF
          BUF04C

          Recommended Positive Controls

            Histology Controls

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