CD36 Antibody (PrecisionAbTM Antibody) | OTI3F4

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CD36 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI3F4 gallery image 1

Western blot analysis of whole cell lysates probed with CD36 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 156 second exposure. Arrow points to CD36 (molecular weight 86 kDa)

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  • Mouse anti CD36
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    Mouse anti human CD36 antibody recognizes CD36 antigen, also known as collagen type I receptor, thrombospondin receptor, fatty acid translocase (FAT), platelet glycoprotein IV (GP4; GPIV), glycoprotein IIIb (GP3B) or PAS IV. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00162WBdatasheet pdfdatasheet pdf100 µl
      VMA00162
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human CD36 antibody recognizes CD36, also known as CD36 antigen (collagen type I receptor, thrombospondin receptor), GPIIIB, PAS IV, PAS-4 protein, cluster determinant 36, fatty acid translocase, glycoprotein IIIb, leukocyte differentiation antigen CD36, platelet glycoprotein 4, platelet glycoprotein IV and scavenger receptor class B, member 3.

        The protein encoded by the CD36 gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in CD36 cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the same protein have been found for CD36 (provided by RefSeq, Jul 2008).

        Mouse anti Human CD36 antibody detects a band of 86 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti CD36 detects a band of approximately 86 kDa in U-937 cell lysates.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography from ascites.
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length recombinant human CD36 (NP_000063) produced in HEK293T cells
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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