CD321 Antibody | M.Ab.F11

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CD321 Antibody | M.Ab.F11 gallery image 1

Published customer image:
Mouse anti Human CD321 antibody, clone F11 (MCA2270) used for the visualization of CD321 expression on microvascular endothelial cells in vitro by immunofluorescence.
Image caption:
Immunocytochemical JAM-A tight junction staining in primary HBMEC is reduced under barrier-disturbing pro-inflammatory conditions.
Cells were either left unstimulated or treated with TNF-α 10 ng/mL and IFN-γ 100 U/mL for 24 h.
Immunocytochemical staining was performed with primary antibodies against JAM-A (M.Ab.F11). Representative for 5 independent experiments with different HBMEC preparations. Bar = 25μm.

From: Haarmann A, Deiß A, Prochaska J, Foerch C, Weksler B, Romero I, et al. (2010)
Evaluation of Soluble Junctional Adhesion Molecule-A as a Biomarker of Human Brain Endothelial Barrier Breakdown.
PLoS ONE 5(10): e13568.

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CD321 Antibody | M.Ab.F11 gallery image 2

Published customer image:
Mouse anti Human CD321 antibody, clone F11 (MCA2270) used for the evaluation of CD321 expression on microvascular endothelial cells in vitro by flow cytometry.
Image caption:
In contrast to ICAM-1, JAM-A overall surface expression levels on HBMEC do not change upon pro-inflammatory stimulation.
Flow cytometric analysis of Accutase™-dissociated single cell suspensions of primary HBMEC after stimulation as in Figure 1. JAM-A was stained with M.Ab.F11 and a rabbit polyclonal antibody from Zymed. Staining against ICAM-1 served as a positive control. In parallel, unstimulated and stimulated HBMEC were stained with matched isotype control antibodies. Filled histograms represent isotype stainings, open histograms JAM-A and ICAM-1 stainings. Representative for 5 independent experiments with different HBMEC preparations.

From: Haarmann A, Deiß A, Prochaska J, Foerch C, Weksler B, Romero I, et al. (2010)
Evaluation of Soluble Junctional Adhesion Molecule-A as a Biomarker of Human Brain Endothelial Barrier Breakdown.
PLoS ONE 5(10): e13568.

Enlarge
CD321 Antibody | M.Ab.F11 gallery image 3

Published customer image:
Mouse anti Human CD321 antibody, clone F11 (MCA2270) used for the evaluation of CD321 expression on microvascular endothelial cells in vitro by western blotting.
Image caption:
Pro-inflammatory stimulation of HBMEC induces JAM-A dissociation from the actin cytoskeleton.
A Primary HBMEC were either left unstimulated or treated with TNF-α 10 ng/mL or IFN-γ 100 IU/mL alone or in combination. Cell protein extracts were generated with a Nonidet-P40 based cell lysis buffer and subjected to Western blot analysis. JAM-A was stained with M.Ab.F11. Staining of the same, peroxidase-inactivated membranes with a rabbit antibody against β-actin served as a loading control. B N-deglycosylation of JAM-A with increasing concentrations of PNGase F for 2 h. A rabbit polyclonal antibody against JAM-A (Zymed) was used for the detection of JAM-A. The asterisk represents N-glycosylated, the open circle N-declycosylated JAM-A. Representative experiments out of at least 5 independent experiments with different EC preparations for each subpanel of the figure are shown.

From: Haarmann A, Deiß A, Prochaska J, Foerch C, Weksler B, Romero I, et al. (2010)
Evaluation of Soluble Junctional Adhesion Molecule-A as a Biomarker of Human Brain Endothelial Barrier Breakdown.
PLoS ONE 5(10): e13568.

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  • Mouse anti Human CD321
  • Mouse anti Human CD321
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    M.Ab.F11
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2270F, FN*, IF, IP, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA2270
    MCA2270TF, IF, IP, WBdatasheet pdfdatasheet pdf25 µg
    MCA2270T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD321 antibody, clone F11 recognises human Junctional adhesion molecule A, also known as CD321, JAM-1, JAM-A, Platelet F11 receptor, Platelet adhesion molecule 1 or PAM-1. CD321 is a 272 amino acid ~35 kDa single pass type 1 transmembrane glycoprotein expressed by endothelium, epithlial tissues and some leukocytes (Ostermann et al. 2002). CD321 was originally identified as the F11 receptor expressed on platelets (Kornecki et al. 1990).

      Mouse anti Human CD321 antibody, clone F11 is able to activate platelets, leading to aggregation and granule secretion (Kornecki et al. 1990, Naik et al. 1995).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Human platelet membranes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      interspecies interaction between organisms
      tight junction assembly
      tight junction
      integral to membrane
      blood coagulation
      leukocyte migration
      inflammatory response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunofluorescence
      Immunoprecipitation
      Western Blotting1/1001/1000
      Functional Assays(1)
      (1)
      This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunofluorescence
      Immunoprecipitation
      Western Blotting1/1001/1000
      Functional Assays(1)
      (1)
      This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ml
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      MCA2270 detects a band of approximately 35-40kDa in platelet cell lysates.
    • Western Blotting
      MCA2270 detects a band of approximately 35-40kDa in platelet cell lysates.
    • Instructions For Use
    • Instructions For Use

    Additional CD321 Antibody Formats

    Formats Clone Applications Sizes available
    CD321 Antibody : Purified M.Ab.F11 F, FN*, IF, IP, WB 0.2 mg | 25 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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        Useful Reagents

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              References

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