CD314 antibody | 1D11
Mouse anti Human CD314
- Product Type
- Monoclonal Antibody
- Clone
- 1D11
- Isotype
- IgG1
- Specificity
- CD314
Mouse anti Human CD314 antibody, clone 1D11 recognizes CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding toCD314 results in NK cell activation and potent co-stimulation of effector T cells. Mouse anti Human CD314 antibody, clone 1D11 is reported to inhibit T cell recognition of MICA (Bauer et al. 1999). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- NKL cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised RBF/DnJ mice were fused with cells of the p3 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/200 | |
Immunohistology - Frozen | |||
Immunoprecipitation |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control |
Source Reference
-
Bauer, S. et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA.
Science. 285 (5428): 727-9.
References for CD314 antibody
-
Wu, J. et al. (2000) DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells.
J Exp Med. 192:1059-68. -
Groh, V. et al. (2001) Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells.
Nat Immunol. 2 (3): 255-60. -
Roberts, A.I. et al. (2001) NKG2D receptors induced by IL-15 costimulate CD28-negative effector CTL in the tissue microenvironment.
J Immunol. 167: 5527-30. -
Gumperz, J. et al. (2002) Functionally distinct subsets of CD1d-restricted natural killer T cells revealed by CD1d tetramer staining.
J Exp Med. 195:625-36. -
Wu, J. et al. (2002) T cell antigen receptor engagement and specificity in the recognition of stress-inducible MHC class I-related chains by human epithelial gamma delta T cells.
J Immunol. 169:1236-40. -
Jinushi, M. et al. (2003) Autocrine/paracrine IL-15 that is required for type I IFN-mediated dendritic cell expression of MHC class I-related chain A and B is impaired in hepatitis C virus infection.
J Immunol. 171 (10): 5423-9. -
Groh, V. et al. (2003) Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
Proc Natl Acad Sci U S A. 100:9452-7 -
Das, H. et al. (2004) Mechanisms of Vdelta1 gammadelta T cell activation by microbial components.
J Immunol. 172 (11): 6578-86.
View The Latest Product References
-
Holmen, C. et al. (2007) Anti endothelial cell autoantibodies selectively activate SAPK/JNK signalling in Wegener's granulomatosis.
J Am Soc Nephrol. 18: 2497-508. -
Sugita, J. et al. (2010) Differential effects of interleukin-12 and interleukin-15 on expansion of NK cell receptor-expressing CD8+ T cells.
Ann Hematol. 89: 115-20. -
Tanaka, J. et al. (2012) Expansion of NK cells from cord blood with antileukemic activity using GMP-compliant substances without feeder cells.
Leukemia. 26 (5): 1149-52. -
Matzner, P. et al. (2013) Resilience of the immune system in healthy young students to 30-hour sleep deprivation with psychological stress.
Neuroimmunomodulation. 20: 194-204. -
Voigt, J. et al. (2014) Human natural killer cells acting as phagocytes against Candida albicans and mounting an inflammatory response that modulates neutrophil antifungal activity.
J Infect Dis. 209 (4): 616-26. -
Tahrali, I. et al. (2019) CD3(-)CD56(+) NK cells display an inflammatory profile in RR-MS patients.
Immunol Lett. 216: 63-9.
Further Reading
-
Groh, V. et al. (2003) Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
Proc Natl Acad Sci U S A. 100 (16): 9452-7.
- Synonyms
- NKG2D
- RRID
- AB_567174
- UniProt
- P26718
- Entrez Gene
- KLRC4-KLRK1
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0030101 natural killer cell activation
- GO:0031295 T cell costimulation
MCA2405
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