Negative Isotype Controls
- Recombinant Human CD31 antigen expressed in HEK293 cells comprises the extracellular domain of CD31, otherwise known as PECAM-1, a member of the Ig-related superfamily of adhesion molecules, expressed by endothelial cells, platelets, granulocytes and some lymphocytes, and plays a key role as a mediator of leucocyte-endothelial cell adhesion and leucocyte transendothelial migration, during inflammatory responses. CD31 has been linked with various inflammatory disorders including atherosclerosis, rheumatoid arthritis (RA) and multiple sclerosis (MS).
Purified recombinant protein - lyophilised
Reconstitute with 500ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant CD31 expressed in HEK293 cells
Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 μg/ml.
>97% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
Recombinant human CD31 comprises the extracellular domain of CD31, consisting of 572 amino acid residues. Monomeric glycosylated CD31 migrates at approximately 80-95kDa by SDS-PAGE, under reducing conditions.
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
3 months from date of reconstitution.
For research purposes only
Recommended Secondary Antibody
Recommended Negative Isotype Control
Recommended Positive Controls