CD305 Antibody

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Human lymph node cell lysate probed with Goat anti Human CD305 (AHP2257)

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  • Goat anti Human CD305
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2257E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2257
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human CD305 antibody recognises human Leukocyte-associated immunoglobulin-like receptor 1, also known as CD305 or LAIR-1. CD305 is a 287 amino acid ~30kDa, single pass typeI transmembrane glycoprotein, expressed by natural killer cells, monocytes, dendritic cells, and T cells, and also by other hematopoeitic cells including B cells, but is not expressed by platelets or erythrocytes.

      Although structurally related to other killer cell inhibitory receptors, CD305 does not appear to recognize HLA class I, providing a novel mechanism of HLA class I-independent NK cell regulation. CD305 has been shown to inhibit proliferation, cellular activation and inflammation, and to induce apoptosis in myeloid leukemia cell lines, and found to be expressed at abnormally low levels in naive B cells from HIV-1 infected patients.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human CD305 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence C-NDTEDVSQASPSE from the internal region of CD305 (NP_002278.1; NP_068352.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1000
      Western Blotting1.03.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Goat anti Human CD305 detects a band of approximately 28kDa in Human lymph node cell lysates.
    • Instructions For Use

    Additional CD305 Antibody Formats

    Formats Applications Sizes available
    CD305 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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