Mouse anti Human CD3:FITC/HLA-CLASS 2:RPE Dual color reagent is designed to permit the simultaneous enumeration of CD3 and HLA Class 2 expressing cells. It is particularly useful in the assessment of HLA Class 2 expression on T lymphocytes. Clone UCHT1 recognizes the human CD3 cell surface antigen, expressed by T lymphocytes. Clone WR18 recognises a common determinant on the beta chain of the human HLA Class 2 molecules HLA-DR, DP and DQ. This antigen is expressed on B lymphocytes, monocytes and on activated T lymphocytes.
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood