CD3/CD8 | UCHT1/LT8

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CD3/CD8 | UCHT1/LT8 gallery image 1

DC003 staining of human lymphocytes

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  • Mouse anti Human CD3:FITC/CD8:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Dual Color Reagent
  • Clone
    UCHT1/LT8
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    DC003Fdatasheet pdfdatasheet pdf50 Tests
    DC003
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    References
    Reviews
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    • Mouse anti Human CD3:FITC/CD8:RPE dual color reagent may be used to simultaneously identify CD3 and CD8 positive cells and to identify populations of lymphocytes that co-express these antigens.

      Clone UCHT1 recognises the human CD3 antigen, which is expressed by all mature T cells, while clone LT8 recognises the human CD8 antigen, a ~32 kDa dimer expressed by a subset of T cells with cytotoxic/suppressor activity, and more weakly by NK cells.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD3/CD8 Formats

    Formats Clone Applications Sizes available
    CD3/CD8 : FITC/RPE UCHT1/LT8 F 50 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1:FITC/Mouse IgG1:RPE Negative ControlDC01250 TestsF
        DC012

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              References

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