Mouse anti Human CD3:FITC/CD16:RPE dual color reagent has been developed to allow the simultaneous enumeration of Tcells and NK cells, as well as the subset of CD3+ve, CD16+ve lymphocytes.
CD3 is a complex cell-surface glycoprotein expressed by all mature T lymphocytes. This complex comprises five different polypeptide chains, with molecular weights of ~16-28 kDa. CD16 is a ~50-65 kDa glycoprotein expressed by NK cells, some T cells and granulocytes. It exists in two forms, a transmembrane protein expressed on NK cells and a GPI-linked form expressed on granulocytes. CD16 functions as a low affinity receptor for IgG, and is also known as FcRIII.
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised
Reconstitute with 0.5ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
1. Beverley, P.C.L. and Callard, R.E. (1981) Distinctive functional characteristics of human T lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.Eur. J. Immunol. 11: 329-334.
2. Edburg, J.C. et al. (1989) Human Fc gamma RIII (CD16). Isoforms with distinct allelic expression, extracellular domains, and membrane linkages on polymorphonuclear and natural killer cells.J. Immunol. 143: 1642-1649.