CD3/CD16/CD56/CD45/CD19 | UCHT1 / LNK16 / MEM-188 / F10-89-4 / LT19

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CD3/CD16/CD56/CD45/CD19 | UCHT1 / LNK16 / MEM-188 / F10-89-4 / LT19 gallery image 1

Human peripheral lymphocytes stained with Multi-Colour Human CD3/CD16+CD56/CD45/CD19 flow kit (4C007)

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  • CD3/CD16/CD56/CD45/CD19 | UCHT1 / LNK16 / MEM-188 / F10-89-4 / LT19 thumbnail image 1
  • Four-Color Human CD3/CD16+CD56/CD45/CD19 Flow Kit
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  • Product Type
    Four Color Reagent
  • Clone
    UCHT1 / LNK16 / MEM-188 / F10-89-4 / LT19
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4C007Fdatasheet pdfdatasheet pdf50 Tests/0.5ml
    4C007
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Four-Color Human CD3/CD16+CD56/CD45/CD19 Flow Kit can be used for single-step identification of T-cells (CD3+), B-cells (CD3-CD19+) and NK cells (CD3-CD16+ and/or CD56+), useful in the study of NK-mediated cytotoxicity against malignant and virally infected cells and the characterization and monitoring of immunodeficiency and autoimmune diseases. CD45 is a 180-240 kDa complex glycoprotein and member of the protein-tyrosine phosphatase family, otherwise known as leucocyte common antigen (LCA), and is expressed on all haematopoietic cells with the exception of mature erythrocytes and platelets. CD45 is a complex molecule existing in a number of isoforms including CD45RA and CD45RO. Clone F10-89-4 reacts with all isoforms. CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, θ), consisting of two heterodimers (εγ, εδ) and one homodimer (θθ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the ~20 kDa CD3ε chain. CD19 is a member of the immunoglobulin super family, which acts as a critical signal transduction molecule and regulator of B-cell development, differentiation and activation. CD19 is expressed on follicular dendritic cells and B-cells, from earliest recognizable B-lineage cells to B-cell blasts, but is lost on maturation to plasma cells. As such, CD19 is a commonly used pan B-cell marker. CD16/CD56, used in combination, facilitate the identification of natural killer (NK) cell sub-populations which express CD16+/CD56+, CD16+/CD56- or CD16-/CD56+ but lack CD3 and T-cell receptor (TCR) expression. There are important functional differences between these NK cell subsets. CD16-/CD56+(bright) cells, a minority in peripheral blood but representing the majority of NK cells in secondary lymphoid tissues, exhibit a high cytokine production capacity but are less effective at mediating antibody-dependent cellular cytotoxicity (ADCC) and natural NK cytotoxicity than other subsets.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD3/CD16/CD56/CD45/CD19 Formats

    Formats Clone Applications Sizes available
    CD3/CD16/CD56/CD45/CD19 : 4 Color UCHT1 / LNK16 / MEM-188 / F10-89-4 / LT19 F 50 Tests/0.5ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Flow Cytometry Absolute Count Standard™FCSC58020 TestsF
          FCSC580
          Erythrolyse Red Blood Cell Lysing Buffer (10x)BUF04B100 Test/20mlF
          BUF04B
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          Erythrolyse Red Blood Cell Lysing Buffer (10x)BUF04C500 Tests/100mlF
          BUF04C

          Recommended Positive Controls

            Histology Controls

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