CD292 Antibody | AbD01564

CD292 Antibody | AbD01564 gallery image 1

Detection of BMPR-1A (extracellular domain) when probed with 1ug/ml HCA004 followed by Goat anti Human IgG F(ab')2: HRP (0500-0099). A: 50ng of antigen, non-reduced; B: 50ng of antigen, reduced; C: 15ng - 100ng of antigen, non-reduced

  • CD292 Antibody | AbD01564 thumbnail image 1
  • Human anti CD292
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
    HuCAL Fab monovalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA004E, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Human anti Human CD292 antibody, clone AbD01564 recognizes human CD292, also known as bone morphogenetic protein receptor type 1A (BMPR-1A) and does not cross-react with human BMPR-1B.

      The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR-1A and BMPR-1B and the type II receptor BMPR2.These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. BMPR-1A is the receptor for BMP-2 and BMP-4. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kDa and type II receptors of about 70-80 kDa. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signalling, whereas type I receptors require their respective type II receptors for ligand binding.

      In humans, mutations in the BMPR-1A gene are responsible for juvenile polyposis syndrome, juvenile intestinal polyposis, and Cowden disease. Human anti Human CD292 antibody inhibits BMP-2 mediated stimulation of C2C12 cells.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      BovineExpected from Sequence
      DogExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using Streptactin affinity chromatography. The antibody is tagged with a strepII-tag (NWSHPQFEK) at the C-terminus of the antibody heavy chain-liquid.
    • Reconstitution
    • Preparation
      Streptactin affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Human BMPR-1A extracellular domain, recombinant expressed in E. coli (amino acid residues 24-152, molecular weight 14.2 kDa)
    • Activity
      Activity was tested by indirect ELISA: recombinant purified BMPR-1A (5 μg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA004 (2 μg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate, 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above back-ground.
    • Purity
      Purity was tested by SDS-PAGE and Coomassie-staining of a 2 µg sample.
    • Affinity
      kD = 2.2 nM
      The monovalent intrinsic affinity of HCA004 was measured by BIAcore on the immobilized BMPR-1A extracellular domain.
    • Approx. Protein Concentrations
      Antibody concentration 0.5 mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      immune response
      transforming growth factor beta receptor activity
      integral to membrane
      BMP signaling pathway
      positive regulation of pathway-restricted SMAD protein phosphorylation
      transforming growth factor beta receptor signaling pathway
      protein serine/threonine kinase activity
      SMAD binding
      positive regulation of osteoblast differentiation
      positive regulation of bone mineralization
      protein phosphorylation
      plasma membrane
      protein homodimerization activity
      positive regulation of SMAD protein import into nucleus
      ATP binding
      metal ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      Blot data kindly provided by Professor Sebald, University of Wuerzburg, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays
      Western Blotting2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD292 Antibody Formats

    Formats Clone Applications Sizes available
    CD292 Antibody : Purified AbD01564 E, FN, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:Alk. Phos.0500-01000.5 mlE, WB
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


              Further Reading

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