CD289 Antibody | 5G5

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CD289 Antibody | 5G5 gallery image 1

Published customer image:
Mouse anti Human CD289 antibody, clone 5G5 used for the evaluation of TLR9 expression in canine polynoprphonuclear leukocytes using flow cytometry.
Image caption:
Percentage of TLR positive PMNs in different disease categories. Boxes contain values from the 1st to the 3rd quartile, lines inside boxes indicate median values, endpoints of vertical lines display the 5th to 95th percentile and · represent the outliers. Asterisks indicate statistically significant differences (* P?<0.05; ** P?<0.01; *** P?<0.005). BM, bacterial meningitis; CSF, cerebrospinal fluid; Healthy, healthy dogs; Mix, miscellaneous diseases of the nervous system; MUE, meningoencephalitis of unknown etiology; PB, peripheral blood; PMNs, polymorphonuclear cells; Pyo, pyogenic infection; SRMA, steroid-responsive meningitis-arteritis; SRMA A, SRMA Acute; SRMA Th, SRMA under therapy; TLR, Toll-like receptor.

From: Maiolini A, Carlson R, Tipold A. Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 2012 Sep 27;9:226.

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CD289 Antibody | 5G5 gallery image 2

Published customer image:
Mouse anti Human CD289 antibody, clone 5G5 used for the evaluation of TLR9 expression in canine lymphocytes using flow cytometry.
Image caption:
Percentage of TLR positive lymphocytes in different disease categories. Boxes contain values from the 1st to the 3rd quartile, lines inside boxes indicate median values, endpoints of vertical lines display the 5th to 95th percentile and · represents the outliers. Asterisks indicate statistically significant differences (* P?<0.05; ** P?<0.01; *** P?<0.005) BM, bacterial meningitis; CSF, cerebrospinal fluid; Healthy, healthy dogs; Mix, miscellaneous diseases of the nervous system; MUE, meningoencephalitis of unknown etiology; PB, peripheral blood; PMNs, polymorphonuclear cells; Pyo, pyogenic infection; SRMA, steroid-responsive meningitis-arteritis; SRMA A, SRMA Acute; SRMA Th, SRMA under therapy; TLR, Toll-like receptor.

From: Maiolini A, Carlson R, Tipold A. Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 2012 Sep 27;9:226.

Enlarge
CD289 Antibody | 5G5 gallery image 3

Published customer image:
Mouse anti Human CD289 antibody, clone 5G5 used for the evaluation of TLR9 expression in canine monocytes using flow cytometry.
Image caption:
Precentage of TLR positive monocytes in different disease categories. Boxes contain values from the 1st to the 3rd quartile, lines inside boxes indicate median values, endpoints of vertical lines display the 5th to 95th percentile and · represents the outliers. Asterisks indicate statistically significant differences (* P?<0.05; ** P?<0.01; *** P?<0.005). BM, bacterial meningitis; CSF, cerebrospinal fluid; Healthy, healthy dogs; Mix, miscellaneous diseases of the nervous system; MUE, meningoencephalitis of unknown etiology; PB, peripheral blood; PMNs, polymorphonuclear cells; Pyo, pyogenic infection; SRMA, steroid-responsive meningitis-arteritis; SRMA A, SRMA Acute; SRMA Th, SRMA under therapy; TLR, Toll-like receptor.

From: Maiolini A, Carlson R, Tipold A. Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 2012 Sep 27;9:226.

Enlarge
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  • Mouse anti Human CD289
  • Mouse anti Human CD289
  • Mouse anti Human CD289
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5G5
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2265GAC, F *, P, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2265GA
    MCA2265C, F *, P, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA2265
    MCA2265TC, F *, P, WBdatasheet pdfdatasheet pdf25 µg
    MCA2265T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD289 antibody, clone 5G5 recognizes human, canine and murine TLR9, otherwise known as CD289. TLR9 is a member of the Toll-like receptor (TLR) family and functions as a receptor for bacterial DNA (CpG-DNA), playing a fundamental role in pathogen recognition and activation of innate immunity.
      TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.

      In mouse RAW264 cells TLR9 has been shown to be expressed intracellularly, but not at the cell surface.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting1/501/200
      Flow Cytometry(1)1/101/50
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting1/501/200
      Flow Cytometry(1)1/101/50
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting1/501/200
      Flow Cytometry(1)1/101/50
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      MCA2265GA detects a band of approximately 170-180 kDa in RAW264 cell lysates.
    • Western Blotting
      MCA2265 detects a band of approximately 170-180 kDa in RAW264 cell lysates.
    • Western Blotting
      MCA2265 detects a band of approximately 170-180 kDa in RAW264 cell lysates.
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD289 Antibody Formats

    Formats Clone Applications Sizes available
    CD289 Antibody : Purified 5G5 C, F *, P, WB 25 µg | 0.25 mg | 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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              References

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