CD283 Antibody | TLR3.7

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  • Mouse anti Human CD283
  • Mouse anti Human CD283
  • Mouse anti Human CD283
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    TLR3.7
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2267GAF *, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2267GA
    MCA2267F *, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA2267
    MCA2267TF *, IPdatasheet pdfdatasheet pdf25 µg
    MCA2267T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD283 antibody, clone TLR3.7 recognizes human and canine TLR3, otherwise known as CD283. TLR3 is a member of the Toll-like receptor (TLR) family and functions as a receptor for double-stranded (ds) RNA.
      TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.

      Cell surface expression of TLR3 has been reported on MRC-5 fibroblasts (Matsumoto et al.2002). Mouse anti Human CD283 antibody, clone TLR3.7 has been shown to suppress poly (I) : poly C mediated IFN beta production by these cells.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Dogyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Ba/F3 cells transfected with human TLR3 (CD283).
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.2 mg, 25 µgIgG concentration 0.5 mg/ml
      Pack Size: 0.1 mgIgG concentration 0.5mg/ml
      Pack Size: 0.2 mg, 25 µgIgG concentration 0.5 mg/ml
      Pack Size: 0.1 mgIgG concentration 0.5mg/ml
      Pack Size: 0.2 mg, 25 µgIgG concentration 0.5 mg/ml
      Pack Size: 0.1 mgIgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      negative regulation of osteoclast differentiation
      positive regulation of tumor necrosis factor production
      I-kappaB phosphorylation
      positive regulation of interleukin-6 production
      endosome membrane
      positive regulation of interferon-beta production
      positive regulation of interleukin-12 production
      innate immune response
      integral to plasma membrane
      hyperosmotic response
      transmembrane receptor activity
      positive regulation of chemokine production
      defense response to virus
      positive regulation of NF-kappaB import into nucleus
      inflammatory response
      positive regulation of interferon-beta biosynthetic process
      positive regulation of gene-specific transcription from RNA polymerase II promoter
      defense response to bacterium
      endoplasmic reticulum membrane
      positive regulation of inflammatory response
      detection of virus
      positive regulation of interleukin-8 production
      activation of NF-kappaB-inducing kinase activity
      double-stranded RNA binding
      positive regulation of interferon-alpha biosynthetic process
      positive regulation of toll-like receptor signaling pathway
      positive regulation of interferon-gamma biosynthetic process
      toll-like receptor 3 signaling pathway
      MyD88-independent toll-like receptor signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Flow Cytometry(1)1/101/50
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Flow Cytometry(1)1/101/50
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Flow Cytometry(1)1/101/50
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD283 Antibody Formats

    Formats Clone Applications Sizes available
    CD283 Antibody : Purified TLR3.7 F *, IP 25 µg | 0.2 mg | 0.1 mg
    • Copyright © 2016 Bio-Rad

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        Useful Reagents

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              • Application NameReference Images
                Flow Cytometry
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              References

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