CD281 Antibody | GD2.F4

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  • Mouse anti Human CD281
  • Mouse anti Human CD281
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    GD2.F4
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2153GAFdatasheet pdfdatasheet pdf0.1 mg
    MCA2153GA
    MCA2153Fdatasheet pdfdatasheet pdf0.2 mg
    MCA2153
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD281 antibody, clone GD2.F4 recognizes human Toll-like receptor 1, also known as CD281or Toll/interleukin-1 receptor-like protein. CD281 is a 786 amino acid ~90 kDa single pass type 1 transmembrane glycoprotein protein expressed by peripheral blood leukocytes and in tissues such as, spleen and thymus.

      CD281 plays an important role in the innate immune response to microbial pathogens (Netea et al. 2004).

      Mouse anti Human CD281 antibody, clone GD2.F4 does not cross react with CD282 (TLR2), CD284 (TLR4) or TLR4/MD-2.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      CD281 (TLR1) Fc fusion protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice where fused with cells of the NS0/1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      innate immune response
      detection of triacyl bacterial lipopeptide
      macrophage activation
      inflammatory response
      positive regulation of tumor necrosis factor biosynthetic process
      Toll-like receptor 1-Toll-like receptor 2 protein complex
      integral to plasma membrane
      positive regulation of interleukin-6 biosynthetic process
      cellular response to triacyl bacterial lipopeptide
      transmembrane receptor activity
      phagocytic vesicle membrane
      protein heterodimerization activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD281 Antibody Formats

    Formats Clone Applications Sizes available
    CD281 Antibody : Purified GD2.F4 F 0.1 mg | 0.2 mg
    • Copyright © 2016 Bio-Rad

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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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