CD273 Antibody | MIH14

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CD273 Antibody | MIH14 gallery image 1

Staining of human peripheral blood monocytes with Mouse anti Human CD273:Alexa Fluor® 488 (MCA2630A488)

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CD273 Antibody | MIH14 gallery image 2

Staining of human peripheral blood monocytes with Mouse anti Human CD273:FITC (MCA2630F)

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CD273 Antibody | MIH14 gallery image 3

Staining of human peripheral blood monocytes with Mouse anti Human CD273:RPE (MCA2630PE)

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CD273 Antibody | MIH14 gallery image 4

Staining of human peripheral blood monocytes with Mouse anti Human CD273 Alexa Fluor® 647 (MCA2630A647)

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CD273 Antibody | MIH14 gallery image 5

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD273 antibody, clone MIH14 (MCA2630) followed by Histar detection system (STAR3000). Low power

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CD273 Antibody | MIH14 gallery image 6

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD273 antibody, clone MIH14 (MCA2630) followed by Histar detection system (STAR3000). Medium power

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CD273 Antibody | MIH14 gallery image 7

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD273 antibody, clone MIH14 (MCA2630) followed by Histar detection system (STAR3000). High power

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CD273 Antibody | MIH14 gallery image 8

Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD273 antibody, clone MIH14 (MCA2630) followed by Histar detection system (STAR3000). High power

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CD273 Antibody | MIH14 gallery image 9

Immunofluorescence staining of human tonsil cryosection with Mouse nti Human CD273 antibody, clone MIH14 (MCA2630), red in A anti Mouse anti Human CD21 antibody, clone LB21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

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CD273 Antibody | MIH14 gallery image 10

Immunofluorescence staining of human tonsil cryosection with Mouse nti Human CD273 antibody, clone MIH14 (MCA2630), red in A anti Mouse anti Human CD21 antibody, clone LB21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power

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CD273 Antibody | MIH14 gallery image 11

Immunofluorescence staining of human tonsil cryosection with Mouse nti Human CD273 antibody, clone MIH14 (MCA2630), red in A anti Mouse anti Human CD21 antibody, clone LB21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power

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  • Mouse anti Human CD273
  • Mouse anti Human CD273
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MIH14
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2630GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA2630GA
    MCA2630C, Fdatasheet pdfdatasheet pdf0.2 mg
    MCA2630
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD273 antibody, clone MIH14 detects human CD273, also known as B7-DC and Programmed Death Ligand 2 (PD-L2). CD273 is a ~42 kDa member of the immunoglobulin receptor superfamily, and is a ligand for CD279 (PD-1). CD273 is primarily expressed on dendritic cells, and its expression can be induced on inflammatory macrophages when activated by interleukin 4 or Th2 cells.

      CD273 functions to inhibit T-cell proliferation by blocking cell cycle progression and cytokine production, and is particularly important in the maintenance of self tolerance.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Human B7-DC transfected L cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Cells from immunised mice were fused with cells of the P3U1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      plasma membrane
      integral to membrane
      endomembrane system
      immune response
      extracellular region
      receptor activity
      T cell costimulation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
      Human tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD273 Antibody Formats

    Formats Clone Applications Sizes available
    CD273 Antibody : Purified MIH14 C, F 0.2 mg | 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      STAR9B
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human tonsil
              Human tonsil

              References

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