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CD27 antibody | LT27

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Anti Human CD27 Antibody, clone LT27 thumbnail image 1 Anti Human CD27 Antibody, clone LT27 thumbnail image 2 Anti Human CD27 Antibody, clone LT27 thumbnail image 3 Anti Human CD27 Antibody, clone LT27 thumbnail image 4 Anti Human CD27 Antibody, clone LT27 thumbnail image 5 Anti Human CD27 Antibody, clone LT27 thumbnail image 6 Anti Human CD27 Antibody, clone LT27 thumbnail image 7 Anti Human CD27 Antibody, clone LT27 thumbnail image 8 Anti Human CD27 Antibody, clone LT27 thumbnail image 9 Anti Human CD27 Antibody, clone LT27 thumbnail image 10 Anti Human CD27 Antibody, clone LT27 thumbnail image 11 Anti Human CD27 Antibody, clone LT27 thumbnail image 12 Anti Human CD27 Antibody, clone LT27 thumbnail image 13 Anti Human CD27 Antibody, clone LT27 thumbnail image 14 Anti Human CD27 Antibody, clone LT27 thumbnail image 15 Anti Human CD27 Antibody, clone LT27 thumbnail image 16 Anti Human CD27 Antibody, clone LT27 thumbnail image 17 Anti Human CD27 Antibody, clone LT27 thumbnail image 18 Anti Human CD27 Antibody, clone LT27 thumbnail image 19 Anti Human CD27 Antibody, clone LT27 thumbnail image 20 Anti Human CD27 Antibody, clone LT27 thumbnail image 21 Anti Human CD27 Antibody, clone LT27 thumbnail image 22 Anti Human CD27 Antibody, clone LT27 thumbnail image 23 Anti Human CD27 Antibody, clone LT27 thumbnail image 24 Anti Human CD27 Antibody, clone LT27 thumbnail image 25 Anti Human CD27 Antibody, clone LT27 thumbnail image 26 Anti Human CD27 Antibody, clone LT27 thumbnail image 27
Anti Human CD27 Antibody, clone LT27 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD19 (MCA1940F) and A647 conjugated Mouse IgG2a isotype control (MCA929A647). Figure B FITC conjugated Mouse anti Human CD19 (MCA1940F) and A647 conjugated Mouse anti Human CD27 (MCA755A647). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Anti Human CD27 Antibody, clone LT27 gallery image 2
Published customer image:
Mouse anti Human CD27 antibody, clone LT27 (MCA755GA) used for the evaluation of CD27 expression on human B cell subsets by flow cytometry.
Image caption:
Memory B cell sorting strategies.
Subsets of human B cells (CD45+CD19+) were sorted from BM and spleens of vaccinated HIS mice. The strategy 1 (upper part) was used on 3 responder HBsAg-vaccinated animals and gave rise to the following B cell subsets: (i) CD27hiCD38hi, (ii) CD27+CD38lo/intIgD+, and (iii) CD27+CD38lo/intIgD−. The strategy 2 (lower part) was used on 3 responder TT-vaccinated animals and gave rise to the following B cell subsets: (iv) CD27+IgM+IgG−, and (v) CD27+IgM−IgG+. The respective frequency and name (from i to v) of each sorted B cell subsets is indicated in the corresponding flow cytometry dot plot.).

From: Becker PD, Legrand N, van Geelen CMM, Noerder M, Huntington ND, Lim A, et al. (2010)
Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated “Human Immune System” Mice.
PLoS ONE 5(10): e13137.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD27 Antibody, clone LT27 gallery image 3
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and SBV515 conjugated Mouse anti Human CD27 (MCA755SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 4
Figure A. PE-CY5 conjugated Mouse anti Human CD3 (MCA463C). Figure B. PE-CY5 conjugated Mouse anti Human CD3 (MCA463C) and StarBright Violet 670 conjugated Mouse anti Human CD27 (MCA755SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 5
Figure A. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C). Figure B. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C) and StarBright Violet 440 conjugated Mouse anti Human CD27 (MCA755SBV440). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 6
Figure A. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C). Figure B. PECy5.5 conjugated Mouse anti Human CD3 (MCA463C) and StarBright Violet 610 conjugated Mouse anti Human CD27 (MCA755SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 7
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and StarBright Violet 570 conjugated Mouse anti Human CD27 (MCA755SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 8
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515) and StarBright Violet 710 conjugated Mouse anti Human CD27 (MCA755SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 9
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 790 conjugated Mouse anti Human CD27 (MCA755SBV790). All experiments performed on red blood cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 10
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and StarBright Violet 475 conjugated Mouse anti Human CD27 (MCA755SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 11
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790) and StarBright UltraViolet 400 conjugated Mouse anti Human CD27 (MCA755SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 12
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 760 conjugated Mouse anti Human CD27 (MCA755SBV760). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 13
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright UltraViolet 665 conjugated Mouse anti Human CD27 (MCA755SBUV665). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 14
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610) and StarBright UltraViolet 445 conjugated Mouse anti Human CD27 (MCA755SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 15
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700) and StarBright UltraViolet 510 conjugated Mouse anti human CD27 (MCA755SBUV510). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 16
Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and StarBright UltraViolet 575 conjugated Mouse anti Human CD27 (MCA755SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 17
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright UltraViolet 605 conjugated Mouse anti Human CD27 (MCA755SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 18
Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD3 (MCA463SBUV795). Figure B StarBright UltraViolet 795 conjugated Mouse anti Human CD3 (MCA463SBUV795) and StarBright Blue 580 conjugated Mouse anti Human CD27 (MCA755SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 19
Figure A. APC conjugated Mouse anti Human CD3 (MCA6146APC). Figure B. APC conjugated Mouse anti Human CD3 (MCA6146APC) and StarBright Blue 615 conjugated Mouse anti Human CD27 (MCA755SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 20
Figure A. RPE conjugated Mouse anti Human CD3 (MCA6146PE). Figure B. RPE conjugated Mouse anti Human CD3 (MCA6146PE) and StarBright Blue 675 conjugated Mouse anti Human CD27 (MCA755SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 21
Figure A. StarBright UltraViolet 665 conjugated Mouse anti Human CD3 (MCA463SBUV665). Figure B. StarBright UltraViolet 665 conjugated Mouse anti Human CD3 (MCA463SBUV665) and StarBright Blue 765 conjugated Mouse anti Human CD27 (MCA755SBB765). All experiments performed on red blood cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 22
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Blue 810 conjugated Mouse anti Human CD27 (MCA755SBB810). All experiments performed on red blood cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 23
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Yellow 575 conjugated Mouse anti Human CD27 (MCA755SBY575). All experiments performed on red cell lysed Human blood gated on live single cell lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 24
Figure A. StarBright Blue 580 conjugated Mouse anti Human CD3 (MCA463SBB580). Figure B. StarBright Blue 580 conjugated Mouse anti Human CD3 (MCA463SBB580) and StarBright Yellow 720 conjugated Mouse anti Human CD27 (MCA755SBY720). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 25
Figure A. StarBright Violet 475 conjugated Mouse anti Human CD3 (MCA463SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD3 (MCA463SBV475) and StarBright Yellow 605 conjugated Mouse anti Human CD27 (MCA755SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 26
Figure A. StarBright UltraViolet 605 conjugated Mouse anti Human CD3(MCA463SBUV605). Figure B. StarBright UltraViolet 605 conjugated Mouse anti Human CD3 (MCA463SBUV605) and StarBright Yellow 665 conjugated Mouse anti Human CD27 (MCA755SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD27 Antibody, clone LT27 gallery image 27
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Yellow 800 conjugated Mouse anti Human CD27 (MCA755SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD27:FITC

Product Type
Monoclonal Antibody
Clone
LT27
Isotype
IgG2a
Specificity
CD27

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MCA755F
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Mouse anti human CD27, clone LT27, recognizes the human T-cell activation antigen, CD27. This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines.

Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1.0%Bovine Serum Albumin
Immunogen
Normal human blood lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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Description Mouse IgG2a Negative Control:FITC

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References for CD27 antibody

  1. Ravoet, A.M. et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC
    in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. Oxford University Press pp 408-411.
  2. Boxall, S. et al. (2004) Abnormal cell surface antigen expression in individuals with variant CD45 splicing and histiocytosis.
    Pediatr Res. 55 (3): 478-84.
  3. Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
    Vet Immunol Immunopathol. 119: 115-22.
  4. Becker, P.D. et al. (2010) Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated ‘‘Human Immune System’’ Mice
    PLoS One. 5(10). pii: e13137.
  5. van Zelm, M.C. et al. (2007) Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion.
    J Exp Med. 204: 645-55.
  6. Steger, B. et al. (2014) CD4(+)and CD8(+)T-cell reactions against leukemia-associated- or minor-histocompatibility-antigens in AML-patients after allogeneic SCT.
    Immunobiology. 219 (4): 247-60.

Flow Cytometry

RRID
AB_321555
UniProt
P26842
Entrez Gene
CD27
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005576 extracellular region
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0006916 anti-apoptosis
GO:0006917 induction of apoptosis
GO:0007166 cell surface receptor linked signaling pathway
GO:0008588 release of cytoplasmic sequestered NF-kappaB
GO:0016064 immunoglobulin mediated immune response
GO:0043027 caspase inhibitor activity
GO:0045579 positive regulation of B cell differentiation
GO:0046330 positive regulation of JNK cascade

MCA755F

0315 160276

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