This item has been added to your cart.
CD262 Antibody | DJR2-4
BJAB cells stained with Mouse anti Human CD262 (MCA4638T):green. Image courtesy of Dr C. Gillet. University of Strasbourg
Enlarge- Mouse anti Human CD262
-
Product TypeMonoclonal Antibody
-
CloneDJR2-4
-
IsotypeIgG1
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
-
- Mouse anti Human CD262 antibody, clone DJR2-4recognises human CD262, also known as Death receptor 5 (DR5) and TNF-related apoptosis-inducing ligand receptor 2 (TRAIL-R2). It is a ~55 kDa member of the TNF receptor superfamily binding tumor necrosis factor-related apoptosis inducing ligand (TNFSF10, TRAIL or APO-2L). Once activated, this receptor induces a cascade of caspases resulting in apoptosis. FADD, a death domain containing adaptor protein is required for apoptosis mediated by DR5. DR5 is widely expressed in adult and fetal tissues. Staining may be enhanced with the use of a sensitive, three-layer staining technique.
-
Intended Use
-
Target SpeciesHuman
-
Product FormPurified IgG - liquid
-
Reconstitution
-
PreparationPurified IgG prepared by affinity chromatography
-
Preservative Stabilisers
0.09% Sodium Azide (NaN3) -
ImmunogenExtracellular domain of DR5-human IgG1 Fc fusion protein.
-
Purity
-
Approx. Protein ConcentrationsIgG concentration 0.5mg/ml
-
Reagents In The Kit
-
Preparing The Antibody
-
Test Principle
-
Buffer SolutionPhosphate buffered saline
-
StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.
-
GO Termscell surface receptor linked signaling pathwayactivation of NF-kappaB-inducing kinase activitycaspase activator activityinduction of apoptosis via death domain receptorsactivation of caspase activitypositive regulation of I-kappaB kinase/NF-kappaB cascadereceptor activityactivation of pro-apoptotic gene productsTRAIL bindingintegral to membraneplasma membrane
-
UniProt
-
Entrez Gene
-
Acknowledgements
-
RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Flow Cytometry 1/10 1/20 Immunofluorescence - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- Flow CytometryUse 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- ELISA
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western Blotting
- Instructions For Use
Additional CD262 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| CD262 Antibody : Purified | DJR2-4 | F, IF | 25 µg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
Recommended Negative Isotype Control
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | MCA928 |
Useful Reagents
Recommended Positive Controls
Histology Controls
You may also be interested in...
Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Ordering
Payment methods we support:
- Invoice / Purchase Order
- Credit card
Follow us on
|
|
|
|
|
Also accessible on: |
||||
|
||||