Goat anti Human CD262 antibody recognizes CD262, otherwise known as DR5/TRAIL-R2, a broadly expressed death domain containing receptor and member of the TNF-receptor superfamily, which acts as a mediator for CD253/TRAIL induced apoptosis in a variety of tumor and transformed cells, but not in normal cells. Expression of CD262 is regulated by tumor suppressor p53, and CD262 is highly expressed by tumour cell lines. Studies suggest that the death domain containing adaptor protein FADD, is required for CD262-mediated apoptosis, and that FADD recruits caspase-8 to the activated receptor.Goat anti Human CD262 antibody is suitable for use in immunocytochemistry.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antiserum to human CD262 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
0.1% Sodium Azide (NaN3)
Synthetic peptide APQQKRSSPSEGLCPPGHHISEDGRDC corresponding to amino acids 68-94 of the extracellular domain of human CD262.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
This product is suitable for use in indirect ELISA applications.