CD262 Antibody

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Paraffin embedded human spleen stained with Goat anti human CD262 (AHP1806)

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  • Goat anti Human CD262
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1806E, F, Pdatasheet pdfdatasheet pdf0.1 mg
    AHP1806
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human CD262 antibody recognizes CD262, otherwise known as DR5/TRAIL-R2, a broadly expressed death domain containing receptor and member of the TNF-receptor superfamily, which acts as a mediator for CD253/TRAIL induced apoptosis in a variety of tumor and transformed cells, but not in normal cells. Expression of CD262 is regulated by tumor suppressor p53, and CD262 is highly expressed by tumour cell lines. Studies suggest that the death domain containing adaptor protein FADD, is required for CD262-mediated apoptosis, and that FADD recruits caspase-8 to the activated receptor.Goat anti Human CD262 antibody is suitable for use in immunocytochemistry.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human CD262 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.1% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide APQQKRSSPSEGLCPPGHHISEDGRDC corresponding to amino acids 68-94 of the extracellular domain of human CD262.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      TRAIL binding
      caspase activator activity
      plasma membrane
      activation of caspase activity
      integral to membrane
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      cell surface receptor linked signaling pathway
      activation of pro-apoptotic gene products
      induction of apoptosis via death domain receptors
      receptor activity
      activation of NF-kappaB-inducing kinase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/75,000
      Flow Cytometry
      Immunohistology - Paraffin1/150

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
      This product is suitable for use in indirect ELISA applications.
    • Immunohistology
    • Histology Positive Control Tissue
      Spleen
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD262 Antibody Formats

    Formats Applications Sizes available
    CD262 Antibody : Purified E, F, P 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Spleen

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