CD256 Antibody | D05-1D4

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  • Mouse anti Human CD256
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    D05-1D4
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5994GAEdatasheet pdfdatasheet pdf0.1 mg
    MCA5994GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD256 antibody, clone D05-1D4 recognizes human CD256, also known as APRIL and TNFSF13. CD256, a 17 kDa soluble protein produced from the 32 kDa type II transmembrane protein by cleavage with furin, is a member of the tumor necrosis factor ligand superfamily (TNFLSF).

      CD256 and its receptor (TNFRSF17/BCMA) are both important for B-cell development (Langat et al. 2008) . It may also be implicated in the regulation of tumor cell growth and monocyte/macrophage-mediated immunological processes. CD256 is expressed in cancers of the colon, thyroid and lymphoid tissues and on monocytes and macrophages (Haiat et al. 2006).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from serum free tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      E. coli derived recombinant protein corresponding to aa 105-250 of human CD256
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      tumor necrosis factor receptor binding
      nucleoplasm
      positive regulation of cell proliferation
      signal transduction
      extracellular space
      mRNA metabolic process
      cytokine activity
      membrane
      immune response
      positive regulation of isotype switching to IgA isotypes
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD256 Antibody Formats

    Formats Clone Applications Sizes available
    CD256 Antibody : Purified D05-1D4 E 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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