CD256 Antibody

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CD256 Antibody gallery image 1

Western blot analysis of whole cell lysates from Jurkat cells probed with Rabbit anti Human CD256 (AHP953) also known as April at 1(A), 2(B) and 4(C) ug/ml

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CD256 Antibody gallery image 2

Immunohistochemical staining of human prostate with Rabbit anti Human CD256 (AHP953)

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  • CD256 Antibody thumbnail image 1
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  • Rabbit anti Human CD256
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP953P, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP953
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human CD256 antibody recognizes Tumor necrosis factor ligand superfamily member 13, also known as CD256, A proliferation-inducing ligand, APRIL, TNF- and APOL-related leukocyte expressed ligand 2 or TNF-related death ligand 1.

      CD256 is a member of the tumour necrosis factor family, expressed in B cells and several tumour cells. The protein is involved in stimulating B and T cell proliferation, triggering humoral immune responses, activating NF-kappaB and inducing cell death. CD256 is thought to play a role in autoimmune diseases and cancer.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human CD256 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      Synthetic peptide corresponding to amino acids 196 to 210 of human CD256.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      cytokine activity
      positive regulation of isotype switching to IgA isotypes
      extracellular space
      nucleoplasm
      mRNA metabolic process
      membrane
      positive regulation of cell proliferation
      signal transduction
      tumor necrosis factor receptor binding
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/100
      Western Blotting1/2501/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP953 detects a band approximately 27kD in Jurkat cell lysates
    • Instructions For Use

    Additional CD256 Antibody Formats

    Formats Applications Sizes available
    CD256 Antibody : Purified P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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