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CD253 Antibody

CD253 Antibody gallery image 1

Formalin fixed, paraffin embedded meatstatic carcinoma of lymph node from human breast stained with Rabbit anti Human CD253 (AHP1215) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0)

CD253 Antibody gallery image 2

Formalin fixed, paraffin embedded meatstatic carcinoma of lymph node from human breast stained with Rabbit anti Human CD253 (AHP1215) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0)

  • CD253 Antibody thumbnail image 1
  • CD253 Antibody thumbnail image 2
  • Rabbit anti Human CD253
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1215E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human CD253 antibody recognizes human Tumor necrosis factor ligand superfamily member 10 otherwise known as TRAIL, CD253 or Apo2L. CD253 is a 281 amino acid type II transmembrane glycoprotein and member of the tumour necrosis factor superfamily, which induces apoptosis in tumour and transformed cells, but not normal cells.

      Activation of apoptosis by CD253 occurs through binding to the death-signaling receptors DR4 (TRAILR1) and DR5 (TRAILR2), leading to formation of the death-inducing signaling complex (DISC) and the triggering of a proteolytic caspase cascade. There are however decoy receptors known as DcR1 (TRAILR3) and DcR2 (TRAILR4) which competitively bind to CD253 and are unable to initiate apoptosis.

      CD253 also plays a role in immune surveillance, acting as a mediator of monocyte, NK cell, dendritic cell and T cell anti-tumour and anti-viral cytotoxicity.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
    • Preparation
    • Antiserum Preparation
      Antisera to human CD253 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography against immobilized antigen.
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant human CD253 (PHP125)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      integral to plasma membrane
      extracellular space
      immune response
      induction of apoptosis by extracellular signals
      soluble fraction
      activation of caspase activity
      activation of pro-apoptotic gene products
      metal ion binding
      cell-cell signaling
      tumor necrosis factor receptor binding
      signal transduction
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays150ng/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)0.25ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Metastatic carcinoma of lymph node from breast tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD253 Antibody Formats

    Formats Applications Sizes available
    CD253 Antibody : Purified E, FN, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Metastatic carcinoma of lymph node from breast tissue
                • Product Images

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                • CD253 Antibody thumbnail image 2

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