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CD25 Antibody | YTH906.9HL

CD25 Antibody | YTH906.9HL gallery image 1

Staining of PHA stimulated human PBMCs with Rat anti Human CD25 followed by Goat F(ab')2 anti Rat IgG:FITC (STAR69)

  • CD25 Antibody | YTH906.9HL thumbnail image 1
  • Rat anti Human CD25
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA350GAFdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rat anti Human CD25, clone YTH906.9HL specifically recognizes human IL-2R alpha chain (IL2RA), otherwise known as CD25, a ~55 kDa type I transmembrane protein which acts as a receptor for interleukin-2 (IL-2).

      CD25 is expressed by activated T and B lymphocytes, macrophages and osteoblasts, and by some thymocytes and myeloid precursors, and can be used in histology as a marker for hairy cell leukemia and systemic mastocytosis. CD4+CD25+ cells are known to act as regulatory T cells (Tregs), high levels of which are associated with advanced stage in esophageal and gastric cancer (Kono et al. 2005).

      Genetic variations in CD25 are associated with susceptibility to the multifactorial disorder diabetes mellitus insulin-dependent type 10 (IDDM10), characterized by susceptibility to ketoacidosis in the absence of insulin therapy, and the clinical manifestations polydipsia, polyphagia and polyuria, and long term eye, kidney, and nerve complications.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Activated human T lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunised DA rat were fused with the rat Y3/Ag.1.2.3 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      interleukin-2 receptor activity
      cell proliferation
    • UniProt
    • Entrez Gene
    • Acknowledgements
      The isolation and characterisation of this cell line was carried out with the help of funds supplied by the Medical Reseach Council, United Kingdom.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD25 Antibody Formats

    Formats Clone Applications Sizes available
    CD25 Antibody : Purified YTH906.9HL F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative ControlMCA6006GA0.1 mgF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • CD25 Antibody | YTH906.9HL thumbnail image 1


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