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CD25 Antibody | BC96

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  • Mouse anti Human CD25:RPE-Cy5
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2808CFdatasheet pdfdatasheet pdf100 Tests/2ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human CD25 antibody, clone BC96 recognizes the human CD25 cell surface antigen, also known as the Interleukin-2 receptor subunit alpha or TAC antigen.

      CD25 is derived from a 272 amino acid precursor to yield a 251 amino acid monomeric receptor of ~53kDa which acts as the low affinity receptor for IL-2. CD25 can also form a heterodimer with CD122 to form the high affinity receptor for IL-2. CD25 is expressed by activated lymphocytes (Jackson et al. 1990, Butcher et al. 1990). Defects in CD4+/ CD25+ reglatory T cells have been implicated in the pathogenesis of autoimmune diabetes (Lindley et al. 2005) and other autoimmune conditions (Sakaguchi et al. 1995).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Cynomolgus monkeyyes
      Rhesus Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to RPE-Cy5 - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC.


      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
    • GO Terms
      interleukin-2 receptor activity
      integral to membrane
      cell proliferation
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Cy® and CyDye® are registered trademarks of GE Healthcare
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 20ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD25 Antibody Formats

    Formats Clone Applications Sizes available
    CD25 Antibody : RPE-Cy5 BC96 F 100 Tests/2ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          Human SeroblockBUF070A50 TestF

          Recommended Positive Controls

            Histology Controls


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