CD243 Antibody | JSB-1

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  • Mouse anti Human CD243
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    JSB-1
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA857HC, P *datasheet pdfdatasheet pdf1 ml
    MCA857H
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD243 antibody, clone JSB-1 recognizes human CD243, also known as Multidrug resistance protein 1 or P-glycoprotein-1 encoded by the ABCB1 gene. CD243 is a 1280 amino acid multi-pass membrane glycoprotein consisting of two homologous subunits bearing a hydrophobic N-terminal domain, six trans-membrane domains and a C-terminal hydrophilic domain containing a nucleotide binding site. The two sub-units are joined by a 60 amino acid linker domain (Ho et al. 2003). CD243 has a predicted molecular mass of 141.5 kDa and apparent MW of ~190 kDa in Western blotting of human embryonal kidney cell line transfected with the MRP1 gene (Akan et al. 2005) . CD243 is widely expressed being found in liver, kidney, small intestine and brain (Koepsell 1998).

      CD243 plays a critical role in the development of ‘multi-drug resistance’ during chemotherapy where it appears to control rated of efflux of chemotherapeutic drugs from cells (Scheper et al. 1988). CD243 also appears to play a significant role on the development of gastrointestinal disease (Ho et al> 2003), including conditions such as IBD13 or Inflammatory Bowel disease 13 (Walker-Smith et al. 2009, Brant et al. 2003).

      Mouse anti Human CD243 antibody, clone JSB-1 has been shown to detect CD243 by Western blotting in C5, a colchcine resistant CHO derived cell line, lysates (Jetté et al. 1993) also in BeWo cell and placental trophoblast lysates(Ushigome et al. 2000) . Mouse anti human CD243 has also been used successfully for the detection of P-glycoprotein in teleost fish using immunohistochemistry (Hemmer et al. 1995).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Concentrated Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Multi drug resistant chinese hamster ovary cell line, CHrC5
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with the cells of the SP2/0 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      apical plasma membrane
      membrane fraction
      integral to membrane
      xenobiotic-transporting ATPase activity
      ATP binding
      Golgi membrane
      cell surface
      intercellular canaliculus
      protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen1/101/20
      Immunohistology - Paraffin(1)1/101/20
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Liver, kidney or lung
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD243 Antibody Formats

    Formats Clone Applications Sizes available
    CD243 Antibody : Concentrate JSB-1 C, P * 1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Liver, kidney or lung

              References

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