CD235a Antibody | E4

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  • Mouse anti Human CD235a
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    E4
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2190SC, F, Hdatasheet pdfdatasheet pdf2 ml
    MCA2190S
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD236a antibdy, clone E4 recognizes the human glycophorin A, also known as CD235a, MN sialoglycoprotein, PAS-2 or Sialoglycoprotein.

      CD235a is a 131 amino acid ~36 kDa glycoprotein expressed exclusively by cells of erythrocyte lineage. Mouse anti Human CD236a antibdy, clone E4 bind to an epitope between residues 27-39 and shows no cross-reaction with CD235b and does not recognise trypsinized cells.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      interspecies interaction between organisms
      integral to membrane
      plasma membrane
      membrane fraction
      identical protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Haemagglutination1/101/100
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Blood smear
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD235a Antibody Formats

    Formats Clone Applications Sizes available
    CD235a Antibody : S/N E4 C, F, H 2 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgM Negative ControlMCA692100 TestsF
        MCA692

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Blood smear

              References

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