CD230 Antibody

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CD230 Antibody gallery image 1

Creutzfeldt-Jakob Disease brain showing immunohistochemical staining of prion plaque with AHP498T at 1:200 dilution in formalin-fixed, paraffin-embedded section of cerebral cortex

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  • Goat anti Human CD230 (N-Terminal)
  • Goat anti Human CD230 (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP498E, P, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP498
    AHP498TPdatasheet pdfdatasheet pdf10 µl
    AHP498T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human CD230 antibody recognizes human Major prion protein, also known as PrP27-30, CD230, or PrP33-35C. CD230 is a 253 amino acid ~27 kDa GPI anchored membrane protein. CD230 is found in large amounts in the brain of patients with transmissible spongiform encephalopathies (TSEs) or prion diseases, fatal infectious neurodegenerative diseases of humans and animals. These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioural changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques.

      CD230. also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties.

      Goat anti Human CD230 (N-Terminal) antibody has been shown to stain PrPSc plaques in formalin fixed sections of CJD brain. It also reacts with PrPc.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Sheepyes
      Hamsteryes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human PrP27-30 were raised by repeated immunisation of goats with highly purified antigen.
    • Preservative Stabilisers
      Pack Size: 0.1 ml
      0.1%Sodium Azide
      Pack Size: 10 µl
      0.09%Sodium Azide
      Pack Size: 0.1 ml
      0.1%Sodium Azide
      Pack Size: 10 µl
      0.09%Sodium Azide
    • Immunogen
      Synthetic peptide corresponding to amino acids 79-97 of the N-terminus of human prion protein
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/30,000
      Immunohistology - Paraffin1/200
      Western Blotting1/2000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/30,000
      Immunohistology - Paraffin1/200
      Western Blotting1/2000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Histology Positive Control Tissue
      CJD brain
    • Histology Positive Control Tissue
      CJD brain
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD230 Antibody Formats

    Formats Applications Sizes available
    CD230 Antibody : Serum E, P, WB 10 µl | 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              CJD brain
              CJD brain

              References

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