CD22 Antibody | Mc64-12

//www.bio-rad-antibodies.com/human-cd22-antibody-mc64-12-mca1380ft.html?RenderPageSpecific=1
CD22 Antibody | Mc64-12 gallery image 1

Staining of human peripheral blood lymphocytes with Mouse anti Human CD22:Biotin (MCA1380B)

Enlarge
CD22 Antibody | Mc64-12 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD22:FITC (MCA1380F)

Enlarge
CD22 Antibody | Mc64-12 gallery image 3

Staining of human peripheral blood lymphocytes with Mouse anti Human CD22:RPE (MCA1380PE)

Enlarge
  • CD22 Antibody | Mc64-12 thumbnail image 1
  • CD22 Antibody | Mc64-12 thumbnail image 2
  • CD22 Antibody | Mc64-12 thumbnail image 3
  • Mouse anti Human CD22:FITC
  • Mouse anti Human CD22:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    Mc64-12
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1380PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1380PE
    MCA1380FTFdatasheet pdfdatasheet pdf25 µg
    MCA1380FT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD22 antibody, clone Mc64-12 recognizes the human CD22 cell surface antigen, a ~130/140 kDa glycoprotein expressed by cells of B lymphocyte lineage.

      CD22 is initially expressed cytoplasmically and subsequently on the cell surface during B cell maturation. It is lost upon terminal differentiation to plasma cells.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Sheepyes
      Bovineyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%
      1% Sodium Azide
      Bovine Serum Albumin
      Inert Bulking Reagent
    • Immunogen
      CD22 isolated from Daudi cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of reconstitution.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNEAT

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD22 Antibody Formats

    Formats Clone Applications Sizes available
    CD22 Antibody : RPE Mc64-12 F 100 Tests
    CD22 Antibody : FITC Mc64-12 F 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...