CD205 Antibody | MG38

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CD205 Antibody | MG38 gallery image 1

Staining of KM-H2 cells with Mouse anti Human CD205 (MCA2258)

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CD205 Antibody | MG38 gallery image 2

Staining of KM-H2 cells with Mouse anti Human CD205: FITC (MCA2258F)

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CD205 Antibody | MG38 gallery image 3

Staining of KM-H2 cells with Mouse anti Human CD205:RPE (MCA2258PE)

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CD205 Antibody | MG38 gallery image 4

Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD205 antibody, clone MG38 followed by the Histar detection system (STAR3000). Low power

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CD205 Antibody | MG38 gallery image 5

Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD205 antibody, clone MG38 followed by the Histar detection system (STAR3000). Medium power

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CD205 Antibody | MG38 gallery image 6

Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD205 antibody, clone MG38 followed by the Histar detection system (STAR3000). High power

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CD205 Antibody | MG38 gallery image 7

Immunofluorescence staining for a human tonsil cryosection with Mouse anti Human CD205 antibody, clone MG38 (MCA2258), red in A and Mouse anti Human CD21, clone LB21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

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CD205 Antibody | MG38 gallery image 8

Immunofluorescence staining for a human tonsil cryosection with Mouse anti Human CD205 antibody, clone MG38 (MCA2258), red in A and Mouse anti Human CD21, clone LB21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power

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CD205 Antibody | MG38 gallery image 9

Immunofluorescence staining for a human tonsil cryosection with Mouse anti Human CD205 antibody, clone MG38 (MCA2258), red in A and Mouse anti Human CD21, clone LB21 (MCA1195), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power

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CD205 Antibody | MG38 gallery image 10

Customer published image:MR and DC-SIGN expression by DC is increased after ECM treatment. A, Following 48 h of culture in FN or LMN-coated plates (both 10 µg/ml), DC were stained for MR and DC-SIGN expression and analyzed by flow cytometry. Filled histograms (gray) represent isotype controls. B, The endocytic receptors MR and DC-SIGN were blocked using mannan before addition of dextran-FITC and analysis by flow cytometry. Filled histograms (gray) represent cells without dextran-FITC and results depict one representative out of three. C, The uptake of specific MR and DC-SIGN ligands by ECM-treated DC was also evaluated by flow cytometry. The sulphated sugar SO4-3-galactose-FITC is specifically bound by MR and Lewis-X-FITC by DC-SIGN. Filled histograms (gray) represent cells incubated without ligand. Results show one representative out of three independent experiments.

From: García-Nieto S, Johal RK, Shakesheff KM, Emara M, Royer P-J, et al. (2010) Laminin and Fibronectin Treatment Leads to Generation of Dendritic Cells with Superior Endocytic Capacity. PLoS ONE 5(4): e10123.

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  • Mouse anti Human CD205:FITC
  • Mouse anti Human CD205:Low Endotoxin
  • Mouse anti Human CD205
  • Mouse anti Human CD205:FITC
  • Mouse anti Human CD205:RPE
  • Mouse anti Human CD205
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MG38
  • Isotype
    IgG2b
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2258FF *datasheet pdfdatasheet pdf0.1 mg
    MCA2258F
    MCA2258C, F *, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA2258
    MCA2258ELC, F *, IPdatasheet pdfdatasheet pdf0.5 mg
    MCA2258EL
    MCA2258PEF *datasheet pdfdatasheet pdf100 Tests
    MCA2258PE
    MCA2258FTF *datasheet pdfdatasheet pdf25 µg
    MCA2258FT
    MCA2258TC, F *, IPdatasheet pdfdatasheet pdf25 µg
    MCA2258T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD205 antibody, clone MG38 recognizes human CD205, a ~205 kDa cell surface glycoprotein that is also known as DEC-205. CD205 is a multilectin receptor which in humans is predominantly expressed by dendritic cells. >Mouse anti Human CD205 antibody, clone MG38 stains mature monocyte - derived dendritic cells and weakly stains some peripheral blood mononuclear cells. Clone MG38 also stains cortical epithelium in the thymus.

      Mouse anti Human CD205 antibody, clone MG38 is routinely tested in flow cytometry on the KM-H2 cell line.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      CR-Fn II fusion protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised DEC-205 knock-out mice were fused with cells of the SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      immune response
      sugar binding
      inflammatory response
      receptor activity
      integral to plasma membrane
      endocytosis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/5
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunoprecipitation
      Flow Cytometry(1)
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunoprecipitation
      Flow Cytometry(1)1/251/50
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/5
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/10
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunoprecipitation
      Flow Cytometry(1)1/251/50
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of suggested working dilution 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD205 Antibody Formats

    Formats Clone Applications Sizes available
    CD205 Antibody : FITC MG38 F * 0.1 mg | 25 µg
    CD205 Antibody : RPE MG38 F * 100 Tests
    CD205 Antibody : Purified MG38 C, F *, IP 25 µg | 0.2 mg
    CD205 Antibody : Low Endotoxin MG38 C, F *, IP 0.5 mg
    • Copyright © 2016 Bio-Rad

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              • Application NameReference Images
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              References

              Further Reading

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