CD20 antibody | 10F381
0.5 μg of Human Anti-CD20 Antibody, MCA6091 (rituximab biosimilar, research grade) and rituximab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Size exclusion chromatography (SEC) of Human Anti-CD20 Antibody, MCA6091 (rituximab biosimilar, research grade) and rituximab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl; flow rate: 200 μl/min; inj. volume [μl]: 2; temp.: 25 °C; detection: 280 nm; sample: mAb (0.5-5 mg/ml).
A microtiter plate was coated overnight with Human Anti-Rituximab Antibody (clone AbD18423, HCA186) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of rituximab (therapeutic reference product) or Human Anti-CD20 Antibody, MCA6091 (rituximab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Rituximab Antibody (clone AbD18423_hIgG1, HCA201) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®.
Human anti CD20 (Rituximab Biosimilar)
- Product Type
- Monoclonal Antibody
- Clone
- 10F381
- Isotype
- IgG1
- Specificity
- CD20
Antibody Quality and Performance Guarantee
| Human Anti-CD20 antibody, clone 10F381 is a research grade biosimilar of the monoclonal antibody drug rituximab. This product is supplied in a research grade format, i.e. in PBS buffer with preservative, it is not manufactured or formulated in the same way as the therapeutic reference product. A preservative free version is available on request. This product is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic rituximab antibody clone 10F381. This antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-rituximab antibodies, for example HCA186 and HCA201. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-rituximab antibody in IgG1 format as a reference standard, for example HCA201. Rituximab is a chimeric monoclonal antibody with murine variable region and human constant domains. It targets CD20, a protein found on the surface of B cells. Binding of the drug to its target triggers cell death through a number of mechanisms, including apoptosis, complement activation and cell-mediated cytotoxicity. Rituximab has been approved for the treatment of B cell malignancies, transplant rejection and autoimmune disorders. This product is NOT FOR THERAPEUTIC USE. View a summary of supporting anti-rituximab antibody products. |
- Target Species
- Human
- Product Form
- Human IgG1 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Source
- HEK
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% ProClin 300
- Immunogen
- Human lymphoblastoid cell line SB
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Rituximab | HCA061 | E | 0.1 mg |
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| Description | Human anti Rituximab | ||||||
| Human anti Rituximab | HCA062 | E | 0.1 mg |
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| Description | Human anti Rituximab | ||||||
| Human anti Rituximab | HCA186 | E | 0.1 mg |
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| Description | Human anti Rituximab | ||||||
| Human anti Rituximab | HCA201 | E | 0.1 mg |
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| Description | Human anti Rituximab | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
| Rat anti Rituximab | MCA2260 | E F | 0.2 mg |
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| List Price | Your Price | ||||||
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| Description | Rat anti Rituximab | ||||||
| Rat anti Rituximab:FITC | MCA2260F | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Rat anti Rituximab:FITC | ||||||
| Rat anti Rituximab:HRP | MCA2260P | E | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Rat anti Rituximab:HRP | ||||||
- Licensed Use
- For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
MCA6091
151687
153012
159920
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