CD20 Antibody | 10F381

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CD20 Antibody | 10F381 gallery image 1

Purity assessment by SDS-PAGE

0.5 μg of Human Anti-CD20 Antibody, MCA6091 (rituximab biosimilar, research grade) and rituximab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.

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Monodispersity assessment by HPLC-SEC

Size exclusion chromatography (SEC) of Human Anti-CD20 Antibody, MCA6091 (rituximab biosimilar, research grade) and rituximab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl; flow rate: 200 μl/min; inj. volume [μl]: 2; temp.: 25 °C; detection: 280 nm; sample: mAb (0.5-5 mg/ml).

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Rituximab bridging ELISA for pharmacokinetic (PK) assay development

A microtiter plate was coated overnight with Human Anti-Rituximab Antibody (clone AbD18423, HCA186) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of rituximab (therapeutic reference product) or Human Anti-CD20 Antibody, MCA6091 (rituximab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Rituximab Antibody (clone AbD18423_hIgG1, HCA201) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®.

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  • Human anti CD20 (Rituximab Biosimilar)
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-CD20 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug rituximab (Rituxan) for research use. It can be used in bioanalytical PK and ADA assays and for studying biological pathways affected by the drug.
    • Product Type
      Monoclonal Antibody
    • Clone
      10F381
    • Isotype
      IgG1
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA6091Edatasheet pdfdatasheet pdf0.2 mg
      MCA6091
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human Anti-CD20 Antibody is a research grade biosimilar of the monoclonal antibody drug rituximab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic rituximab antibody clone 10F381.

        This antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-rituximab antibodies, for example HCA186 and HCA201. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-rituximab antibody in IgG1 format as a reference standard, for example HCA201.

        Rituximab is a chimeric monoclonal antibody with murine variable region and human constant domains. It targets CD20, a protein found on the surface of B cells. Binding of the drug to its target triggers cell death through a number of mechanisms, including apoptosis, complement activation and cell-mediated cytotoxicity. Rituximab has been approved for the treatment of B cell malignancies, transplant rejection and autoimmune disorders.

        This product is NOT FOR THERAPEUTIC USE.

        View a summary of supporting anti-rituximab antibody products.
      • Intended Use
      • Target Species
        Human
      • Product Form
        Human IgG1 (kappa) antibody - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.02% Proclin 300
      • Immunogen
        Human lymphoblastoid cell line SB
      • Purity
        >98% by SDS PAGE
      • Approx. Protein Concentrations
        IgG concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
      • Acknowledgements
      • Licensed Use
        For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional CD20 Antibody Formats

      Formats Clone Applications Sizes available
      CD20 Antibody : Purified 10F381 E 0.2 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Rat anti Rituximab:FITCMCA2260F0.1 mgF
            MCA2260F
            Human anti Rituximab:HRPHCA061P0.1 mgE
            HCA061P
            Human anti Rituximab:HRPHCA062P0.1 mgE
            HCA062P
            Rat anti Rituximab:HRPMCA2260P0.1 mgE
            MCA2260P
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            Human anti RituximabHCA0610.1 mgE
            HCA061
            Human anti RituximabHCA0620.1 mgE
            HCA062
            Human anti RituximabHCA1860.1 mgE
            HCA186
            Human anti RituximabHCA2010.1 mgE
            HCA201
            Rat anti RituximabMCA22600.2 mgE, F
            MCA2260
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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