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CD195 Antibody (PrecisionAb^TM Antibody)

CD195 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with CD195 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 300 second exposure. Arrow points to CD195 (molecular weight 37 kDa)

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Goat anti CD195

CD195 Antibody with Control Lysate

CD195 antibody recognizes C-C chemokine receptor type 5, also known as C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, or HIV-1 fusion coreceptor. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type

PrecisionAb™ Polyclonal
Isotype

Polyclonal IgG
Format

Purified

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
VPA00044	WB			100 µl	VPA00044
VPA00044KT	WB			2 Westerns	VPA00044KT

Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

References

Reviews

-

Goat anti Human CD195 antibody recognizes human CD195, also known as C-C chemokine receptor type 5, CCR5 or C-C CKR-5. CD195 is a 353 amino acid ~37 kDa multiple pass transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, preferentially expressed by a subset of memory Th1 (type 1 helper T) cells.

CD195 acts as a high-affinity receptor for several chemotactic chemokines, including MIP-1 alpha (macrophage inflammatory protein 1 alpha), MIP-1 beta, and RANTES (regulated on activation normal T expressed and secreted protein). CD195 is also a major co-receptor for HIV-1 infection, with variations in the CD195 gene, influencing the rate of progression into AIDS (Choe et al. 1996).

Goat anti Human CD195 antibody detects CD195 as a 37 kDa band, in a number of human cell lysates, by western blotting under reducing conditions. Cross reactivity with rodent cell lines is also observed.

Target Species

Human
Western Blotting

Goat anti CD195 detects a band of approximately 37 kDa in A549 cell lysates.
Goat anti CD195 detects a band of approximately 37 kDa in A549 cell lysates.
Species Cross-Reactivity

Target Species Cross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG - liquid
Purified IgG - liquid
Preparation

100μl Goat polyclonal antibody purified by affinity chromatography
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution

Phosphate buffered saline
Phosphate buffered saline
Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
0.5 % Bovine Serum Albumin
0.09% Sodium Azide (NaN₃)
0.5 % Bovine Serum Albumin
Immunogen

Synthetic peptide YQVSSPIYDINYYTSEPCQKINVKQIAA corresponding to amino acids 3-30 within the N-terminal region of human CD195.
Instructions for Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Lysate Composition

400μg A549 lysate lyophilized in RIPA buffer.
Lysate Reconstitution

- If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage

Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life

As supplied, 12 months from date of despatch.
As supplied, 12 months from date of despatch.
Acknowledgements

PrecisionAb™ is a trademark of Bio-Rad Laboratories.
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory

For research purposes only
UniProt

P51681
Related reagents
Entrez Gene

CCR5
Related reagents

Target Species	Cross Reactivity
Mouse
Rat

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Yes	No	Min Dilution	Max Dilution
Western Blotting				1/1000

Application Name	Yes	No	Min Dilution	Max Dilution
Western Blotting				1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

Recommended Secondary Antibody

Description	Product Code	Pack Size	Applications	List Price	Quantity
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C, E, P, WB	STAR88P

Description	Product Code	Pack Size	Applications	List Price	Quantity
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C, E, P, WB	STAR88P

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