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CD195 Antibody (PrecisionAbTM Antibody)
- Goat anti CD195
- CD195 Antibody with Control Lysate
- CD195 Antibody with Control Lysate
CD195 antibody recognizes C-C chemokine receptor type 5, also known as C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, or HIV-1 fusion coreceptor. The antibody has been extensively validated for western blotting using whole cell lysates.
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Product TypePrecisionAb™ Polyclonal
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IsotypePolyclonal IgG
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FormatPurified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00044 | WB |  | | 100 µl | VPA00044 | |
| VPA00044K | WB | | | 10 Westerns | VPA00044K | |
| VPA00044KT | WB | | | 2 Westerns | VPA00044KT |
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
References
Reviews
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- Goat anti Human CD195 antibody recognizes human CD195, also known as C-C chemokine receptor type 5, CCR5 or C-C CKR-5. CD195 is a 353 amino acid ~37 kDa multiple pass transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, preferentially expressed by a subset of memory Th1 (type 1 helper T) cells.
CD195 acts as a high-affinity receptor for several chemotactic chemokines, including MIP-1 alpha (macrophage inflammatory protein 1 alpha), MIP-1 beta, and RANTES (regulated on activation normal T expressed and secreted protein). CD195 is also a major co-receptor for HIV-1 infection, with variations in the CD195 gene, influencing the rate of progression into AIDS (Choe et al. 1996).
Goat anti Human CD195 antibody detects CD195 as a 37 kDa band, in a number of human cell lysates, by western blotting under reducing conditions. Cross reactivity with rodent cell lines is also observed.
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Target SpeciesHuman
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Western BlottingGoat anti CD195 detects a band of approximately 37 kDa in A549 cell lysates.Goat anti CD195 detects a band of approximately 37 kDa in A549 cell lysates.Goat anti CD195 detects a band of approximately 37 kDa in A549 cell lysates.
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Species Cross-Reactivity
N.B. Antibody reactivity and working conditions may vary between species.Target Species Cross Reactivity Mouse 
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Product FormPurified IgG - liquidPurified IgG - liquidPurified IgG - liquid
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Preparation100μl Goat polyclonal antibody purified by affinity chromatographyPack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatographyPack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatographyPack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatographyPack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
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Buffer SolutionPhosphate buffered salinePhosphate buffered salinePhosphate buffered saline
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Preservative Stabilisers0.09% Sodium Azide (NaN3)
0.5 % Bovine Serum Albumin0.09% Sodium Azide (NaN3)
0.5 % Bovine Serum Albumin0.09% Sodium Azide (NaN3)
0.5 % Bovine Serum Albumin -
ImmunogenSynthetic peptide YQVSSPIYDINYYTSEPCQKINVKQIAA corresponding to amino acids 3-30 within the N-terminal region of human CD195.
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Instructions for UsePlease refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
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Lysate Composition400μg A549 lysate lyophilized in RIPA buffer.400μg A549 lysate lyophilized in RIPA buffer.
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Lysate Reconstitution- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol. -
StorageStore undiluted at -20oC, avoiding repeated freeze thaw cycles.Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. -
Shelf LifeAs supplied, 12 months from date of despatch.As supplied, 12 months from date of despatch.As supplied, 12 months from date of despatch.
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AcknowledgementsPrecisionAb™ is a trademark of Bio-Rad Laboratories.PrecisionAb™ is a trademark of Bio-Rad Laboratories.PrecisionAb™ is a trademark of Bio-Rad Laboratories.
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RegulatoryFor research purposes only
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UniProt
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Entrez Gene
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Western Blotting 1/1000 Application Name Yes No Min Dilution Max Dilution Western Blotting 1/1000 Application Name Yes No Min Dilution Max Dilution Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
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Recommended Negative Isotype Control
Useful Reagents
Recommended Positive Controls
Histology Controls
Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies
Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.
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Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
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