CD186 Antibody

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CD186 Antibody gallery image 1

Western blot analysis of CDw186 (also known as CXCR6/Bonzo) in human spleen tissue lysate in the absence (lane A) or presence (lane B) of peptide with AHP569

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CD186 Antibody gallery image 2

Immunocytochemical staining of human spleen with Rabbit anti Human CD186, also known as BONZO (AHP569)

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  • CD186 Antibody thumbnail image 1
  • CD186 Antibody thumbnail image 2
  • Rabbit anti Human CD186
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP569WBdatasheet pdfdatasheet pdf0.1 mg
    AHP569
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human CD186 antibody recognizes the orphan G protein-coupled chemokine receptor CXCR6, also known as CDw186. CXCR6 has been identified as a co-receptor for simian immunodeficiency virus (SIV), HIV-2 and HIV-1. Lymphoid tissues and activated peripheral blood lymphocytes express CXCR6 mRNA.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to CDw186 (NT) were raised by repeated immunisation of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      Peptide corresponding to amino acids 11 to 25 of human CXCR6/CDw186 (NT).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      viral genome replication
      C-C chemokine receptor activity
      coreceptor activity
      integral to plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/500

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD186 Antibody Formats

    Formats Applications Sizes available
    CD186 Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Further Reading

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