CD178 Antibody | 10F2

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CD178 Antibody | 10F2 gallery image 1

Staining of CD178 transfected CHO cells with Mouse anti Human CD178 (MCA2408)

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Staining of CD178 transfected CHO cells with Mouse anti Human CD178 (MCA2408EL)

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CD178 Antibody | 10F2 gallery image 3

Staining of CD178 transfected CHO cells with Mouse anti Human CD178 (MCA2408GA)

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CD178 Antibody | 10F2 gallery image 4

Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 (MCA2409) as a capture reagent and biotinylated Mouse anti Human CD178 (MCA2408) as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma (orange) sample is displayed at 1:2 dilution.

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  • Mouse anti Human CD178
  • Mouse anti Human CD178
  • Mouse anti Human CD178:Low Endotoxin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    10F2
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2408GAE, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2408GA
    MCA2408E, F, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA2408
    MCA2408ELE, F, FN, IPdatasheet pdfdatasheet pdf0.5 mg
    MCA2408EL
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD178 antibody, clone 10F2 recognizes the human CD178 cell surface antigen, a ~40 kDa glycoprotein also known as Fas ligand (CD95L).

      CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form.

      CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.

      Clone 10F2 is reported to block the activity of Fas ligand (Legembre et al. 2002).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%sodium Azide
      0.09%sodium Azide
      None present
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cell-cell signaling
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      integral to plasma membrane
      extracellular space
      induction of necroptosis of activated-T cells
      cytokine activity
      immune response
      activation of pro-apoptotic gene products
      signal transduction
      activation of caspase activity
      negative regulation of angiogenesis
      induction of necroptosis by extracellular signals
      necrotic cell death
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/251/50
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/251/50
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/251/50
      Functional Assays
      Immunoprecipitation
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD178 Antibody Formats

    Formats Clone Applications Sizes available
    CD178 Antibody : Low Endotoxin 10F2 E, F, FN, IP 0.5 mg
    CD178 Antibody : Purified 10F2 E, F, IP 0.1 mg | 0.2 mg
    • Copyright © 2016 Bio-Rad

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