CD169 Antibody | 7-239

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CD169 Antibody | 7-239 gallery image 1

Published customer image:
Mouse anti Human CD169 antibody, clone 7-239 used for the detection of sialoadhein by flow cytometry.
Image caption:
Liposomes bearing 3'-BPCNeuAc ligands bind to and internalized by Sn/CD169 expressing cells. (A) FACS analysis for binding of the naked (blue line) or 3'-BPCNeuAc (red line) liposomes to TSn and Daudi cells that express surface hSn and hCD22 (Siglec-2), respectively. Unstained cells (filled grey) were used as a negative control. Shown are results from 1 of 3 representative experiments followed indicated treatment. (B) Ligand-bound liposomal cargos but not antibodies exhibited time-dependent accumulation in Sn-expressing cells. TSn cells were incubated with fluorescently labeled anti-Sn antibody (Clone 7–239 (Bio-Radh); filled red, left panel) or Sn-targeted liposomes (filled red, right panel) for 5, 20 and 90 min before they were washed with isotonic HBSS buffer prior to FACS analysis. Isotype antibody or naked liposome stained cells were used as negative controls. (C) Internalization of Sn-targeted liposomes by TSn cells. Cells were incubated with fluorescent naked or 3'-BPCNeuAc liposomes for 5, 30 or 60 min at 37°C before they were washed with isotonic HBSS buffer (pH 7) or acid buffer (pH 3.3) prior to FACS analysis to determine levels of the total liposome uptake (membrane bound plus internalized), or internalized liposomes. Cells that were not stained with liposomes were used as a negative control (filled gray). (D) Recycling of Sn between cell surface and inside the cell. Top; unlabeled anti-Sn Ab was incubated with Sn expressing CHO cells at 37°C. Cells were then cooled to 4°C and stained with a labeled secondary Ab after a neutral wash (Red line) or acid wash (Orange line) to detect residual cell surface bound anti-Sn Ab. Isotype control antibody staining is shown as filled histogram. Middle; acid-washed cells from the Top were subjected to a further incubation at 4°C and stained with labeled secondary Ab, showing that no anti-Sn Ab had returned to the cell surface. Bottom; acid-washed cells from the Top were warmed to 37°C to allow internalized anti-Sn Ab to be recycled back to the cell surface. Ab re-appearing on the surface of the cell is detected by staining the cells with labeled secondary Ab. Results shown are representative of at least two independent experiments.

From: Chen WC, Kawasaki N, Nycholat CM, Han S, Pilotte J, et al. (2012) Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169. PLoS ONE 7(6): e39039.

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CD169 Antibody | 7-239 gallery image 2

Published customer image:
Mouse anti Human CD169 antibody used for the detection of sialoadhesin on dendritic cells by flow cytometry.
Image caption:
Siglec-1 is up-regulated in highly trans-infecting LPS mDCs. (A) (Left) Comparative HIV-1 capture of LPS and ITIP mDCs: cells were cultured with HIV-1, washed, and lysed to measure viral p24Gag antigen by ELISA. (Right) Comparative transmission of captured HIV-1 from LPS and ITIP mDCs to a reporter CD4+ cell line. Graphs show mean values and standard error of the means (SEMs) from two independent experiments including cells from six donors. (B) Plot of SIGLEC genes (in open circles), CD86 and DC-SIGN (in grey circles) computing the fold change in LPS mDCs compared to ITIP mDCs, and the average gene expression across all samples. Circle size is inversely proportional to adjusted p values. Highlighted in red are statistically differentially expressed genes. Analysis was performed with DCs from four donors matured in parallel with the different stimuli. (C) Relative quantification of SIGLEC1 mRNA expression levels in distinct DCs analyzed by qRT-PCR. Measurements were normalized using the endogenous control housekeeping gene Beta Glucuronidase. Data show means and SEMs of samples from six donors. (D) Cell surface expression of Siglec-1 in distinct DCs analyzed by FACS with mAb 7–239-PE. (Left graph) Geometric mean fluorescence intensity (MFI) of Siglec-1. (Right graph) Percentage of Siglec-1 positive cells. Data show mean values and SEM from two experiments, including cells from six donors. (Histograms) Representative profiles of Siglec-1 staining in distinct DCs derived from one donor.

From: Izquierdo-Useros N, Lorizate M, Puertas MC, Rodriguez-Plata MT, Zangger N, et al. (2012) Siglec-1 Is a Novel Dendritic Cell Receptor That Mediates HIV-1 Trans-Infection Through Recognition of Viral Membrane Gangliosides. PLoS Biol 10(12): e1001448.

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CD169 Antibody | 7-239 gallery image 3

Published customer image:
Mouse anti Human CD169 antibody, clone 7-239 used for the blocking of sialoadhesin activity on dendritic cells.
Image caption:
Siglec-1 expressed in LPS mDCs capture distinct ganglioside containing vesicles, such as HIV-1 viral-like particles, liposomes, and exosomes. (A) Relative capture of VLPHIV-Gag-eGFP by LPS mDCs that had been pre-incubated with 10 µg/ml of the indicated mAbs or 500 µg/ml of mannan before VLP exposure for 30 min at 37°C. Values are normalized to the level of VLP capture by mock-treated LPS mDCs (set at 100%). Data show mean values and SEMs from three experiments including cells from nine donors. (B) Relative capture of GM1 containing LUVHIV-tRed by LPS mDCs as described in (A). Data show mean values and SEMs from two experiments including cells from six donors. (C) Relative capture of ExosomesDiI by LPS mDCs that had been pre-incubated with 10 µg/ml of the indicated mAbs before exosome exposure for 4 h at 37°C. Values are normalized to the level of exosome capture by isotype-treated LPS mDCs (set at 100%). Data show mean values and SEMs from two experiments including cells from five donors. (D) Capture of VLPHIV-Gag-eGFP by LPS mDCs that had been pre-incubated with decreasing concentrations of a-Siglec-1 mAb 7D2 before VLP exposure for 30 min at 37°C. Titration of a-Siglec-1 mAb 7–239 is shown in Figure S1. Data show mean values and SEMs from three experiments including cells from six donors. (E) Capture of VLPHIV-Gag-eGFP by LPS mDCs that had been pre-incubated with or without 2 µg/ml of a-Siglec-1 mAb 7D2 previously treated or not with at least a 100-fold molar excess of the indicated human recombinant proteins. Of note, Siglec-14 shares 100% of amino acid homology with Siglec-5 in the V-set domain. Data show mean values and SEMs from three experiments including cells from nine donors. (F) Kinetics of VLPHIV-Gag-eGFP capture by iDCs (left graph) and LPS mDCs (right graph) compared to the expression of Siglec-1 over time, assessed after LPS addition to mDCs. Cells were pulsed for 1 h at 37°C with VLPHIV-Gag-eGFP and labeled for Siglec-1 and HLA-DR in parallel at the indicated time points. For comparative purposes, the maximum geometric MFI values obtained by FACS for each donor were set at 100%. Data show mean values and SEMs including cells from three donors. (G) Positive correlation (? = 0.9695) between the geometric MFI of captured VLPs and the mean number of Siglec-1 Ab Binding Sites per cell in different DC subtypes (see also Figure S2 to compare VLP capture capacity among LPS mDCs derived from the same donor). Data show values from three experiments including cells from nine donors.

From: Izquierdo-Useros N, Lorizate M, Puertas MC, Rodriguez-Plata MT, Zangger N, et al. (2012) Siglec-1 Is a Novel Dendritic Cell Receptor That Mediates HIV-1 Trans-Infection Through Recognition of Viral Membrane Gangliosides. PLoS Biol 10(12): e1001448.

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CD169 Antibody | 7-239 gallery image 4

Published customer image:
Mouse anti Human CD169 antibody used for the detection of sialoadhesin on dendritic cells by immunofluorescence.
Image caption:
Siglec-1 captures HIV-1 and traffics with the virus to the same sac-like compartment. (A) Comparative capture of HIV-1 by distinct DCs that had been pre-incubated with 10 µg/ml of the indicated mAbs or 500 µg/ml of mannan for 30 min before viral exposure. Cells were cultured with HIV-1 in the presence of the indicated reagents, washed, and lysed to measure p24Gag by ELISA. Viral binding at 4°C in LPS mDCs is shown in Figure S3. Data show mean values and SEMs from two experiments including cells from six donors. (B) Comparative capture of HIV-1 by distinct DCs first exposed to the virus and then treated with the indicated reagents for 30 min before washing. Cells were lysed and assessed by p24Gag ELISA. Data show mean values and SEMs from two experiments including cells from six donors. (C) Comparative capture of HIV-1 by distinct blood myeloid DCs that had been pre-incubated with 10 µg/ml of the indicated mAbs for 30 min before viral exposure as in panel A. Figure S4 depicts Siglec-1 surface expression levels of blood myeloid cells. Data show mean values and SEMs from two experiments including cells from six donors. (D) Confocal microscopy analysis of LPS mDCs pulsed for 4 h with GM1-containing LUVHIV-tRed, VLPHIV-Gag-Cherry or HIV-1Cherry, fixed, permeabilized, and then stained for Siglec-1 with mAb 7–239-Alexa 488. (Inset) Merge of the bright field and maximun fluorescence intensity (scale bar: 5 µm). (3D images) Isosurface representation of DAPI stained nucleus and maximum fluorescence intensity of the sac-like compartment where particles and Siglec-1 accumulate are shown in a 3D volumetric x-y-z data field. (Bar graphs) Quantification of the percentage of GM1-containing LUVHIV-tRed, VLPHIV-Gag-Cherry or HIV-1Cherry co-localizing with Siglec-1-Alexa 488 7–239 and vice versa, obtained analyzing at least 50 compartments from LPS mDCs of two donors. The mean and standard deviation of the thresholded correlation coefficient of Pearson (obtained considering all the images) were 0.77±0.07, indicating co-localization. See also Movies S1, S2, S3 or Figure S5 to observe the compartment in relation to the plasma membrane or the cytoplasm of the cells. (E) Confocal microscopy analysis showing the sac-like compartment pattern of Siglec-1 in LPS mDCs after internalization of the a-Siglec-1 mAb 7D2. Cells were labeled with the mAb for 30 min at 16°C, revealed with an Alexa 488 secondary Ab, shifted to 37°C for 4 h, and analyzed. (3D image) 3D reconstruction (representative of 69% of the analyzed DCs) was done as in (D). (Inset) Merge of the bright field and maximun fluorescence intensity (scale bar: 5 µm).

From: Izquierdo-Useros N, Lorizate M, Puertas MC, Rodriguez-Plata MT, Zangger N, et al. (2012) Siglec-1 Is a Novel Dendritic Cell Receptor That Mediates HIV-1 Trans-Infection Through Recognition of Viral Membrane Gangliosides. PLoS Biol 10(12): e1001448.

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CD169 Antibody | 7-239 gallery image 5

Published customer image:
Mouse anti Human CD169 antibody used for the detection of sialoadhesin on dendritic cells by immunofluorescence.
Image caption:
Siglec-1 mediates HIV-1 trans-infection to target cells and accumulates at the infectious synapse. (A) HIV-1 transmission from distinct DCs to a reporter CD4+ cell line. DCs were pre-incubated as in Figure 3A, washed, and co-cultured with reporter cells for 48 h. HIV-1 infection of reporter cells was determined by induced luciferase activity in relative light units (RLUs). Data show mean values and SEMs from two experiments including cells from six donors. (B) HIV-1 transmission from distinct DCs first exposed to the virus and then treated with the indicated reagents for 30 min before washing and co-culture with reporter cells. Data show mean values and SEMs from two experiments including cells from six donors. (C) Confocal microscopy analysis of LPS mDCs pulsed with HIV-1Cherry and then co-cultured with CD4+ T cells to reveal Siglec-1 localization. Co-cultures were stained with a-CD4-Alexa 647 mAb to identify the membrane of CD4+ T cells, fixed, permeabilized, and labeled with a-Siglec-1-Alexa 488 7–239 mAb. (Left images) Merge of the bright field and the fluorescence of an x-y plane (scale bar: 5 µm). (Right images) Isosurface representation of DAPI-stained nucleus and maximum fluorescence intensity of the compartment where HIV-1Cherry and Siglec-1 accumulate in the contact zone with a CD4+ T cell, shown in a 3D volumetric x-y-z data field. (D) HIV-1 transmission to reporter cells from distinct blood myeloid DCs that had been pre-incubated with 10 µg/ml of the indicated mAbs for 30 min before viral exposure as in (A). Data show mean values and SEMs from three experiments including cells from twelve donors.

From: Izquierdo-Useros N, Lorizate M, Puertas MC, Rodriguez-Plata MT, Zangger N, et al. (2012) Siglec-1 Is a Novel Dendritic Cell Receptor That Mediates HIV-1 Trans-Infection Through Recognition of Viral Membrane Gangliosides. PLoS Biol 10(12): e1001448.

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CD169 Antibody | 7-239 gallery image 6

Published customer image:
AlexaFluor488 conjugated Mouse anti Human CD169 antibody, clone 7-239 used for the detection of CD169 by immunofluorescence.
Image caption:
Introduction of a di-aromatic motif in CT of CD169 results in endocytosis of HIV-1 particles and attenuation of CD169-mediated trans-infection. (A) Amino acid sequences of the CTs of wild type (WT) CD169 and mutant CD169YF are shown. Alanine to tyrosine mutation at position 1683 (in red) creates a di-aromatic motif, YF (underlined). (B) Western blot analysis for CD169 expression in THP-1/CD169 and THP-1/CD169YF cell lysates. (C) Representative FACS analysis of cell surface expression of CD169 on wild type and YF mutant expressing THP-1 cells. (D) The mean fluorescence intensity of cell surface expression of CD169 on YF mutant expressing THP-1 cells was quantified and normalized to that observed with THP-1/CD169 (wt) cells (set at 100). (E) Cells were incubated with Gag-mCherry VLPs and stained for CD81, CD63 or Lamp1 and nucleus. CD81, CD63 or Lamp1 (green), Gag-mCherry VLP (red) and nucleus (blue). Representative deconvolved images of single slices of cells are shown. Scale bar represents 5 µm. (F) Co-localization between green (CD81, CD63 or Lamp1) and red (VLPs) signals is reported as mean Pearson’s coefficient ± SEM. Each dot represents a single cell. Two-tailed P values were calculated using unpaired t-test in GraphPad Prism 5. ***: P < 0.0001. (G) Cells were challenged with HIV-1, washed and cell-associated p24gag was measured. Virus capture observed with THP-1/CD169YF cells was normalized to that observed with THP-1/CD169 cells (WT; set as 100). (H) Cells challenged with HIV-1/Bal-luc, were washed, co-cultured with CD4+ T cells and lysed at two days post initiation of co-culture for measurement of luciferase activity. The level of virus transmission observed in THP-1/CD169 (wt)—CD4+ T cell co-cultures was set as 100. (I) Efficacy of trans-infection was calculated as trans-infection (luciferase activity) per virus capture (cell-associated p24gag) and is shown relative to that observed with THP-1/CD169 cells (set as 100). The data shown are the means ± SEM of four (D) or six (G to I) independent experiments.

From:Akiyama H, Ramirez N-GP, Gudheti MV, Gummuluru S (2015) CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies. PLoS Pathog 11(3): e1004751.

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AlexaFluor488 conjugated Mouse anti Human CD169 antibody, clone 7-239 used for the detection of CD169 by flow cytometry.
Image caption:
The cytoplasmic tail (CT) of CD169 is dispensable for mediating HIV-1 trans-infection. (A) Sequences of wild type and mutant CD169 CTs. The asterisks represent stop codons introduced into the ORFs of the two CT mutants. (B) Western blot analysis of THP-1 cell lysates expressing either wild type or mutant CD169. (C) Cell surface expression of CD169 on THP-1 cells was measured by flow cytometry. (D) Relative cell surface expression of CD169 CT mutants was quantified and normalized to that observed with THP-1/CD169 cells. (E) Cells were challenged with HIV-1, washed and cell-associated p24gag was measured. The data shown is the virus capture by THP-1/CD169 CT mutants (?CT or ?CT4R) normalized to that observed with THP-1/CD169 cells. (F) THP-1/CD169- or THP-1/CD169 CT mutant-mediated trans-infection was determined by measuring luciferase activity in THP—CD4+ T cell co-cultures 2 days post initiation of co-culture. The data shown is the relative virus transmission by THP-1/CD169 CT mutants (?CT or ?CT4R) to that observed with THP-1/CD169 cells. (G) Efficacy of trans-infection was calculated as trans-infection (luciferase activity) per amount of virus captured (cell-associated p24gag) and normalized to that observed with THP-1/CD169 cells (set as 100). The data shown are the means ± SEM of three (D to F) or four (G) independent experiments. (H) THP-1/CD169 or THP-1/CD169?CT4R cells were incubated with Gag-mCherry VLPs (red), washed, fixed and stained for CD169 (green) and nucleus (blue). Representative deconvolved images of single slices of cells are shown. Scale bar represents 5 µm. WT: THP-1/CD169, ?CT: THP-1/CD169?CT, ?CT4R: THP-1/CD169?CT4R and Vec: empty vector transduced THP-1.

From:Akiyama H, Ramirez N-GP, Gudheti MV, Gummuluru S (2015) CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies. PLoS Pathog 11(3): e1004751.

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AlexaFluor488 conjugated Mouse anti Human CD169 antibody, clone 7-239 used for the detection of CD169 by flow cytometry along with AlexaFluor 647 conjugated Mouse anti Human CD169 antibody, clone 7-239 by fluorescence photoactivated localization microscopy.
Image caption:
Localization of HIV-1 particles in CD169+ deep plasma membrane invaginations in LPS-matured DCs. (A) Representative FACS analysis for CD169 expression on LPS or IFN-a-matured DCs. (B) HIV-1 capture by immature (NT), IFN-a or LPS-matured DCs was determined by measuring cell-associated p24gag in cell lysates. (C) HIV-1 transfer to CD4+ T cells, by immature (NT), IFN-a or LPS-matured DCs was determined by measuring luciferase activity in DC—CD4+ T cell co-cultures. HIV-1 capture and transfer experiments were performed in triplicates with DCs isolated from eight independent donors. The individual dot represents a single donor and the means ± SEM are depicted. (D) LPS or IFN-a-matured DCs were incubated with fluorescent HIV-1 particles (green) and stained for CD169 (red) and nucleus (blue). Representative deconvolved images of single slices of cells are shown. Scale bar represents 5 µm. (E) Representative electron micrographs of LPS or IFN-a-matured DCs incubated with HIV-1. The bottom panels are higher magnification pictures of the area depicted within the highlighted squares in the top panels. Arrows indicate virus particles. Scale bar represents 1 µm for top panels and 500 nm for bottom panels. (F) Cells were incubated with HIV-1 and stained for HIV-1 p24gag (green) and CD169 (red). Cells were imaged by FPALM super resolution microscopy. The top panels represent a single LPS or IFN-a matured DC while the middle panels show cross sections along the a—b line indicated in the top panels. The bottom panels are pictures enlarged from the area depicted within the highlighted (dotted) squares in the middle panels. Scale bars represent 1 µm in the top and middle panels and 500 nm in the bottom panels. LPS: LPS-treated DCs, IFN-a: IFN-a-treated DCs, Immature: immature DCs.

From:Akiyama H, Ramirez N-GP, Gudheti MV, Gummuluru S (2015) CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies. PLoS Pathog 11(3): e1004751.

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AlexaFluor488 conjugated Mouse anti Human CD169 antibody, clone 7-239 used for the detection of CD169 by flow cytometry.
Image caption:
Expression of CD169 in parental and CD169-transduced cell lines.(A) Representative FACS analysis of cell surface expression of CD169 on immature and mature DCs, parental (vector) or CD169-transduced cell lines is shown. (B) Representative FACS analysis of cell surface and total (surface and intracellular) expression of CD169 on wild type and CT mutant expressing THP-1 cells.

From:Akiyama H, Ramirez N-GP, Gudheti MV, Gummuluru S (2015) CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies. PLoS Pathog 11(3): e1004751.

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Published customer image:
AlexaFluor488 conjugated Mouse anti Human CD169 antibody, clone 7-239 used for the detection of CD169 by flow cytometry.
Image caption:
Introduction of a di-aromatic motif in CT of CD169 results in endocytosis of HIV-1 particles. (A) THP-1 cells expressing either wild type (WT) CD169 or CT mutant CD169YF were incubated with saturating amounts (10 µg/ml) of anti-human CD169 antibody or corresponding isotype controls for 30 minutes at 4°C. Cells were washed twice at 4°C and then an aliquot (1x105 cells) was removed and placed on ice (time point 0 min). The remaining cells were then shifted to 37°C for 30 minutes to promote endocytosis. To arrest internalization, cells were transferred to 4°C, and the number of antibody-bound CD169 molecules left at the surface revealed by staining with PE-conjugated goat anti-mouse IgG antibody and analyzed by flow cytometry. The mean fluorescence intensity (MFI) of the isotype controls was subtracted at each time points and MFIs at 30 min were normalized to that observed at 0 min. The data shown is the percent of anti-CD169 antibody remaining at the cell surface 30 minutes post incubation at 37°C and is the mean ± SEM of four independent experiments. (B) Cells were incubated with Gag-mCherry VLPs and stained for plasma membrane bound CD169 (Surface, top panel) or total CD169 (+ Tx100, bottom panel). CD169 (green), Gag-mCherry VLP (red) and nucleus (blue). Representative deconvolved images of single slices of cells are shown. Scale bars represent 5 µm. (C) Co-localization between green (CD169) and red (VLPs) signals is reported as mean Pearson’s coefficient ± SEM. Each dot represents a single cell. Two-tailed P values were calculated using unpaired t-test in GraphPad Prism 5. *: P < 0.05, **: P < 0.01.

From:Akiyama H, Ramirez N-GP, Gudheti MV, Gummuluru S (2015) CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies. PLoS Pathog 11(3): e1004751.

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  • Mouse anti Human CD169:Alexa Fluor® 647
  • Mouse anti Human CD169:RPE
  • Mouse anti Human CD169:FITC
  • Mouse anti Human CD169:Alexa Fluor® 647
  • Mouse anti Human CD169:RPE
  • Mouse anti Human CD169:Alexa Fluor® 488
  • Mouse anti Human CD169
  • Mouse anti Human CD169
  • Mouse anti Human CD169:Low Endotoxin
  • Mouse anti Human CD169:Alexa Fluor® 488
  • Mouse anti Human CD169
  • Mouse anti Human CD169:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    7-239
  • Isotype
    IgG1
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2517FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2517F
    MCA2517GAC *, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2517GA
    MCA2517C *, F, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA2517
    MCA2517ELC *, F, FN, IPdatasheet pdfdatasheet pdf0.5 mg
    MCA2517EL
    MCA2517PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2517PE
    MCA2517A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2517A647
    MCA2517A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2517A488
    MCA2517TC *, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA2517T
    MCA2517FTFdatasheet pdfdatasheet pdf25 µg
    MCA2517FT
    MCA2517A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2517A647T
    MCA2517PETFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2517PET
    MCA2517A488TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2517A488T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD169 antibody, clone 7-239 specifically recognizes human CD169, otherwise known as Sialoadhesin (Sn), a type I transmembrane glycoprotein and prototypic member of the Siglec (sialic acid binding Ig-like lectin) family, designated Siglec-1.

      CD169 is a macrophage-restricted interaction molecule which binds to sialylated ligands on haematopoietic cells, including neutrophils, NK cells, monocytes, a subset of CD8+ T cells and B cells, through recognition of sialic acid in the alpha-2,3- glycosidic linkage. CD169 is highly expressed by dendritic cells and on stromal macrophages in the spleen, bone marrow and lymph nodes and to a lesser extent in liver, lungs and gut, and studies have shown high expression of CD169 on macrophages in the inflammatory conditions rheumatoid arthritis and atherosclerosis.

      Clone 7-239 completely inhibits erythrocyte rosetting around Sn+ transductants.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to ALexa Fluor®647 - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to ALexa Fluor®647 - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to ALexa Fluor®488 - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to ALexa Fluor®488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1.0ml distilled water
      Pack Size: 100 TestsReconstitute with 1.0ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      Pack Size: 100 Tests
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      5%Sucrose
      Pack Size: 25 Tests/0.25ml0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      Pack Size: 100 Tests
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      5%Sucrose
      Pack Size: 25 Tests/0.25ml0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      None present
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
    • Immunogen
      Human Rhinovirus (HRV14) treated monocyte-derived dendritic cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05mg/ml
      IgG concentration 0.1mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/100
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/100
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Functional Assays
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/100
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/100
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human tonsil.
    • Histology Positive Control Tissue
      Human tonsil.
    • Histology Positive Control Tissue
      Human tonsil.
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human tonsil.
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD169 Antibody Formats

    Formats Clone Applications Sizes available
    CD169 Antibody : RPE 7-239 F 100 Tests | 25 Tests/0.25ml
    CD169 Antibody : FITC 7-239 F 25 µg | 0.1 mg
    CD169 Antibody : Purified 7-239 C *, F, IP 0.1 mg | 0.2 mg | 25 µg
    CD169 Antibody : Alexa Fluor® 647 7-239 F 100 Tests/1ml | 25 Tests/0.25ml
    CD169 Antibody : Low Endotoxin 7-239 C *, F, FN, IP 0.5 mg
    CD169 Antibody : Alexa Fluor® 488 7-239 F 100 Tests/1ml | 25 Tests/0.25ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
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      STAR9B
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      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
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      STAR120P
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      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
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      STAR9B
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      HCA036P
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      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
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      STAR120P
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      STAR13B
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      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Low EndotoxinMCA928EL0.5 mgC, F
        MCA928EL
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F

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          BUF070B
          Human SeroblockBUF070A50 TestF
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          Recommended Positive Controls

            Histology Controls

              Human tonsil.
              Human tonsil.
              Human tonsil.
              Human tonsil.

              References

              Further Reading

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