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CD162 Antibody | 3E2.25.5 (PL1)

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  • Mouse anti Human CD162
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  • Product Type
    Monoclonal Antibody
  • Clone
    3E2.25.5 (PL1)
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1726TF, P *datasheet pdfdatasheet pdf25 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human CD162, clone 3E2.25.5 recognises the human CD162 cell surface glycoprotein, a 120 kDa molecule also known as P-Selectin Glycoprotein Ligand I (PSGL-1).

      CD162 is the major counter receptor for P-selectin and is involved in neutrophil attachment and rolling to endothelium. In peripheral blood CD162 is expressed by monocytes, granulocytes, T cells and some B cells.

      Mouse anti Human CD162, clone 3E2.25.5 is a blocking antibody, inhibiting PSGL-1 and P-selectin interaction. Removal of the preservative is recommended prior to use in functional assays - The use of Slide-A-Lyser® dialysis cassette (EQU003) is recommended for this purpose.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared from ascites or tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.1% Bovine Serum Albumin
    • Immunogen
      Human neutrophils.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      membrane fraction
      blood coagulation
      receptor binding
      cellular response to interleukin-6
      bacterial cell surface binding
      integral to plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Paraffin(1)1/200
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD162 Antibody Formats

    Formats Clone Applications Sizes available
    CD162 Antibody : Purified 3E2.25.5 (PL1) F, P * 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays


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